Title |
Structural insight in the inhibition of adherence of F4 fimbriae producing enterotoxigenic Escherichia coli by llama single domain antibodies
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Published in |
Veterinary Research, February 2015
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DOI | 10.1186/s13567-015-0151-x |
Pubmed ID | |
Authors |
Kristof Moonens, Imke Van den Broeck, Emmanuel Okello, Els Pardon, Maia De Kerpel, Han Remaut, Henri De Greve |
Abstract |
Enterotoxigenic Escherichia coli that cause neonatal and post-weaning diarrhea in piglets express F4 fimbriae to mediate attachment towards host receptors. Recently we described how llama single domain antibodies (VHHs) fused to IgA, produced in Arabidopsis thaliana seeds and fed to piglets resulted in a progressive decline in shedding of F4 positive ETEC bacteria. Here we present the structures of these inhibiting VHHs in complex with the major adhesive subunit FaeG. A conserved surface, distant from the lactose binding pocket, is targeted by these VHHs, highlighting the possibility of targeting epitopes on single-domain adhesins that are non-involved in receptor binding. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 30 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 9 | 30% |
Student > Bachelor | 8 | 27% |
Other | 2 | 7% |
Student > Ph. D. Student | 2 | 7% |
Professor | 2 | 7% |
Other | 3 | 10% |
Unknown | 4 | 13% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 8 | 27% |
Biochemistry, Genetics and Molecular Biology | 7 | 23% |
Medicine and Dentistry | 3 | 10% |
Veterinary Science and Veterinary Medicine | 2 | 7% |
Immunology and Microbiology | 2 | 7% |
Other | 3 | 10% |
Unknown | 5 | 17% |