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Changes in cellular microRNA expression induced by porcine circovirus type 2-encoded proteins

Overview of attention for article published in Veterinary Research, April 2015
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2 tweeters

Citations

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15 Dimensions

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17 Mendeley
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Title
Changes in cellular microRNA expression induced by porcine circovirus type 2-encoded proteins
Published in
Veterinary Research, April 2015
DOI 10.1186/s13567-015-0172-5
Pubmed ID
Authors

Jae-Sang Hong, Nam-Hoon Kim, Chang-Yong Choi, Jun-Seong Lee, Dokyun Na, Taehoon Chun, Young Sik Lee

Abstract

Porcine circovirus type 2 (PCV2) is the primary causative agent of postweaning multisystemic wasting syndrome, which leads to serious economic losses in the pig industry worldwide. While the molecular basis of PCV2 replication and pathogenicity remains elusive, it is increasingly apparent that the microRNA (miRNA) pathway plays a key role in controlling virus-host interactions, in addition to a wide range of cellular processes. Here, we employed Solexa deep sequencing technology to determine which cellular miRNAs were differentially regulated after expression of each of three PCV2-encoded open reading frames (ORFs) in porcine kidney epithelial (PK15) cells. We identified 51 ORF1-regulated miRNAs, 74 ORF2-regulated miRNAs, and 32 ORF3-regulated miRNAs that differed in abundance compared to the control. Gene ontology analysis of the putative targets of these miRNAs identified transcriptional regulation as the most significantly enriched biological process, while KEGG pathway analysis revealed significant enrichment for several pathways including MAPK signaling, which is activated during PCV2 infection. Among the potential target genes of ORF-regulated miRNAs, two genes encoding proteins that are known to interact with PCV2-encoded proteins, zinc finger protein 265 (ZNF265) and regulator of G protein signaling 16 (RGS16), were selected for further analysis. We provide evidence that ZNF265 and RGS16 are direct targets of miR-139-5p and let-7e, respectively, which are both down-regulated by ORF2. Our data will initiate further studies to elucidate the roles of ORF-regulated cellular miRNAs in PCV2-host interactions.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Denmark 1 6%
Unknown 16 94%

Demographic breakdown

Readers by professional status Count As %
Researcher 5 29%
Student > Master 4 24%
Student > Ph. D. Student 3 18%
Professor > Associate Professor 3 18%
Professor 1 6%
Other 0 0%
Unknown 1 6%
Readers by discipline Count As %
Agricultural and Biological Sciences 5 29%
Veterinary Science and Veterinary Medicine 4 24%
Biochemistry, Genetics and Molecular Biology 3 18%
Immunology and Microbiology 1 6%
Earth and Planetary Sciences 1 6%
Other 1 6%
Unknown 2 12%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 April 2015.
All research outputs
#3,346,690
of 5,017,282 outputs
Outputs from Veterinary Research
#313
of 472 outputs
Outputs of similar age
#106,664
of 154,825 outputs
Outputs of similar age from Veterinary Research
#20
of 25 outputs
Altmetric has tracked 5,017,282 research outputs across all sources so far. This one is in the 29th percentile – i.e., 29% of other outputs scored the same or lower than it.
So far Altmetric has tracked 472 research outputs from this source. They receive a mean Attention Score of 2.7. This one is in the 29th percentile – i.e., 29% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 154,825 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 26th percentile – i.e., 26% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 25 others from the same source and published within six weeks on either side of this one. This one is in the 20th percentile – i.e., 20% of its contemporaries scored the same or lower than it.