Title |
Conservation of the separase regulatory domain
|
---|---|
Published in |
Biology Direct, April 2018
|
DOI | 10.1186/s13062-018-0210-0 |
Pubmed ID | |
Authors |
Michael Melesse, Joshua N. Bembenek, Igor B. Zhulin |
Abstract |
ᅟ: We report a protein sequence analysis of the cell cycle regulatory protease, separase. The sequence and structural conservation of the C-terminal protease domain has long been recognized, whereas the N-terminal regulatory domain of separase was reported to lack detectable sequence similarity. Here we reveal significant sequence conservation of the separase regulatory domain and report a discovery of a cysteine motif (CxCxxC) conserved in major lineages of Metazoa including nematodes and vertebrates. This motif is found in a solvent exposed linker region connecting two TPR-like helical motifs. Mutation of this motif in Caenorhabditis elegans separase leads to a temperature sensitive hypomorphic protein. Conservation of this motif in organisms ranging from C. elegans to humans suggests its functional importance. This article was reviewed by Lakshminarayan Iyer and Michael Galperin. |
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Geographical breakdown
Country | Count | As % |
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United States | 3 | 30% |
France | 1 | 10% |
Sweden | 1 | 10% |
Unknown | 5 | 50% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 8 | 80% |
Science communicators (journalists, bloggers, editors) | 1 | 10% |
Scientists | 1 | 10% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 14 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 3 | 21% |
Student > Master | 2 | 14% |
Professor | 2 | 14% |
Other | 1 | 7% |
Student > Bachelor | 1 | 7% |
Other | 2 | 14% |
Unknown | 3 | 21% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 9 | 64% |
Agricultural and Biological Sciences | 1 | 7% |
Unknown | 4 | 29% |