Title |
Methylation of free-floating deoxyribonucleic acid fragments in the bronchoalveolar lavage fluid of dogs with chronic bronchitis exposed to environmental tobacco smoke
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Published in |
Irish Veterinary Journal, April 2015
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DOI | 10.1186/s13620-015-0035-4 |
Pubmed ID | |
Authors |
Yoshiki Yamaya, Hiroshi Sugiya, Toshihiro Watari |
Abstract |
The etiology of canine chronic bronchitis (CB) is not completely understood, although exposure to environmental tobacco smoke (ETS) affects the airway inflammatory responses in some dogs with CB. The mechanism by which this occurs is unknown. We investigated the concentrations and methylation rates of free-floating DNA fragments in bronchoalveolar lavage fluid (BALF) from dogs with chronic bronchitis. Based on serum cotinine levels, dogs with CB were divided into 2 groups: dogs that either had or had not been exposed to ETS. Our results demonstrated that the total nucleated cell and macrophage numbers increased in BALF of ETS-exposed dogs with CB. There were no significant differences in DNA concentrations and methylation rates in BALF between the 2 groups. However, 3 out of 8 dogs exposed to ETS had high DNA methylation rates in their BALF samples. Our results suggest that ETS exposure leads to epigenetic modifications of cellular components in BALF in dogs diagnosed with CB. |
X Demographics
Geographical breakdown
Country | Count | As % |
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France | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 13 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Master | 3 | 23% |
Professor | 2 | 15% |
Other | 1 | 8% |
Student > Ph. D. Student | 1 | 8% |
Lecturer | 1 | 8% |
Other | 2 | 15% |
Unknown | 3 | 23% |
Readers by discipline | Count | As % |
---|---|---|
Veterinary Science and Veterinary Medicine | 4 | 31% |
Biochemistry, Genetics and Molecular Biology | 1 | 8% |
Agricultural and Biological Sciences | 1 | 8% |
Computer Science | 1 | 8% |
Economics, Econometrics and Finance | 1 | 8% |
Other | 1 | 8% |
Unknown | 4 | 31% |