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Mendeley readers
Attention Score in Context
| Title |
Comparative proteomic analysis of spermatozoa isolated by swim-up or density gradient centrifugation
|
|---|---|
| Published in |
Reproductive Biology and Endocrinology, April 2015
|
| DOI | 10.1186/s12958-015-0027-y |
| Pubmed ID | |
| Authors |
Stefania Luppi, Monica Martinelli, Elisa Giacomini, Elena Giolo, Gabriella Zito, Rodolfo C Garcia, Giuseppe Ricci |
| Abstract |
Reports about the morphologic and functional characteristics of spermatozoa prepared by density gradient centrifugation (DC) or swim-up (SU) have produced discordant results. We have performed a proteomic comparison of cells prepared by DC and SU providing a molecular insight into the differences between these two methods of sperm cell isolation. Protein maps were obtained by 2-dimensional (2-D) separations consisting of isoelectrofocusing (IEF) from pI 3 to 11 followed by SDS-polyacrylamide gel electrophoresis. 2-D gels were stained with Sypro Ruby. Map images of DC and SU spermatozoa were compared using dedicated software. Intensities of a given spot were considered different between DC and SU when their group mean differed by >1.5-fold (p < 0.05, Anova). No differences were observed for 853 spots, indicating a 98.7% similarity between DC and SU. Five spots were DC > SU and 1 was SU > DC. Proteins present in 3 of the differential spots could be identified. One DC > SU spot contained lactate dehydrogenase C and gamma-glutamylhydrolase, a second DC > SU spot contained fumarate hydratase and glyceraldehyde-3-phosphate dehydrogenase-2, and a SU > DC spot contained pyruvate kinase M1/M2. The differences in protein levels found on comparison of DC with SU spermatozoa indicate possible dissimilarities in their glycolytic metabolism and DNA methylation and suggest that DC cells may have a better capacitation potential. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Spain | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 38 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Iran, Islamic Republic of | 1 | 3% |
| Unknown | 37 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 7 | 18% |
| Student > Ph. D. Student | 6 | 16% |
| Student > Bachelor | 5 | 13% |
| Student > Doctoral Student | 4 | 11% |
| Student > Master | 3 | 8% |
| Other | 5 | 13% |
| Unknown | 8 | 21% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 7 | 18% |
| Agricultural and Biological Sciences | 6 | 16% |
| Medicine and Dentistry | 6 | 16% |
| Veterinary Science and Veterinary Medicine | 2 | 5% |
| Engineering | 2 | 5% |
| Other | 3 | 8% |
| Unknown | 12 | 32% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 May 2015.
All research outputs
#18,409,030
of 22,803,211 outputs
Outputs from Reproductive Biology and Endocrinology
#665
of 973 outputs
Outputs of similar age
#193,441
of 265,270 outputs
Outputs of similar age from Reproductive Biology and Endocrinology
#14
of 24 outputs
Altmetric has tracked 22,803,211 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 973 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 9.9. This one is in the 14th percentile – i.e., 14% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 265,270 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 15th percentile – i.e., 15% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 24 others from the same source and published within six weeks on either side of this one. This one is in the 33rd percentile – i.e., 33% of its contemporaries scored the same or lower than it.