↓ Skip to main content

Identification of phlebotomine sand flies using one MALDI-TOF MS reference database and two mass spectrometer systems

Overview of attention for article published in Parasites & Vectors, May 2015
Altmetric Badge

About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (53rd percentile)
  • Above-average Attention Score compared to outputs of the same age and source (63rd percentile)

Mentioned by

twitter
3 X users
facebook
1 Facebook page

Citations

dimensions_citation
61 Dimensions

Readers on

mendeley
75 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Title
Identification of phlebotomine sand flies using one MALDI-TOF MS reference database and two mass spectrometer systems
Published in
Parasites & Vectors, May 2015
DOI 10.1186/s13071-015-0878-2
Pubmed ID
Authors

Alexander Mathis, Jérôme Depaquit, Vit Dvořák, Holly Tuten, Anne-Laure Bañuls, Petr Halada, Sonia Zapata, Véronique Lehrter, Kristýna Hlavačková, Jorian Prudhomme, Petr Volf, Denis Sereno, Christian Kaufmann, Valentin Pflüger, Francis Schaffner

Abstract

Rapid, accurate and high-throughput identification of vector arthropods is of paramount importance in surveillance programmes that are becoming more common due to the changing geographic occurrence and extent of many arthropod-borne diseases. Protein profiling by MALDI-TOF mass spectrometry fulfils these requirements for identification, and reference databases have recently been established for several vector taxa, mostly with specimens from laboratory colonies. We established and validated a reference database containing 20 phlebotomine sand fly (Diptera: Psychodidae, Phlebotominae) species by using specimens from colonies or field-collections that had been stored for various periods of time. Identical biomarker mass patterns ('superspectra') were obtained with colony- or field-derived specimens of the same species. In the validation study, high quality spectra (i.e. more than 30 evaluable masses) were obtained with all fresh insects from colonies, and with 55/59 insects deep-frozen (liquid nitrogen/-80 °C) for up to 25 years. In contrast, only 36/52 specimens stored in ethanol could be identified. This resulted in an overall sensitivity of 87% (140/161); specificity was 100%. Duration of storage impaired data counts in the high mass range, and thus cluster analyses of closely related specimens might reflect their storage conditions rather than phenotypic distinctness. A major drawback of MALDI-TOF MS is the restricted availability of in-house databases and the fact that mass spectrometers from 2 companies (Bruker, Shimadzu) are widely being used. We have analysed fingerprints of phlebotomine sand flies obtained by automatic routine procedure on a Bruker instrument by using our database and the software established on a Shimadzu system. The sensitivity with 312 specimens from 8 sand fly species from laboratory colonies when evaluating only high quality spectra was 98.3%; the specificity was 100%. The corresponding diagnostic values with 55 field-collected specimens from 4 species were 94.7% and 97.4%, respectively. A centralized high-quality database (created by expert taxonomists and experienced users of mass spectrometers) that is easily amenable to customer-oriented identification services is a highly desirable resource. As shown in the present work, spectra obtained from different specimens with different instruments can be analysed using a centralized database, which should be available in the near future via an online platform in a cost-efficient manner.

X Demographics

X Demographics

The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 75 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Colombia 1 1%
Madagascar 1 1%
Australia 1 1%
Unknown 72 96%

Demographic breakdown

Readers by professional status Count As %
Researcher 19 25%
Student > Master 12 16%
Other 8 11%
Student > Ph. D. Student 7 9%
Student > Bachelor 5 7%
Other 12 16%
Unknown 12 16%
Readers by discipline Count As %
Agricultural and Biological Sciences 22 29%
Biochemistry, Genetics and Molecular Biology 8 11%
Immunology and Microbiology 6 8%
Medicine and Dentistry 5 7%
Veterinary Science and Veterinary Medicine 4 5%
Other 13 17%
Unknown 17 23%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 January 2016.
All research outputs
#12,923,613
of 22,803,211 outputs
Outputs from Parasites & Vectors
#2,187
of 5,461 outputs
Outputs of similar age
#119,268
of 263,961 outputs
Outputs of similar age from Parasites & Vectors
#45
of 128 outputs
Altmetric has tracked 22,803,211 research outputs across all sources so far. This one is in the 42nd percentile – i.e., 42% of other outputs scored the same or lower than it.
So far Altmetric has tracked 5,461 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.7. This one has gotten more attention than average, scoring higher than 58% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 263,961 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 53% of its contemporaries.
We're also able to compare this research output to 128 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 63% of its contemporaries.