Title |
Identification of phlebotomine sand flies using one MALDI-TOF MS reference database and two mass spectrometer systems
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Published in |
Parasites & Vectors, May 2015
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DOI | 10.1186/s13071-015-0878-2 |
Pubmed ID | |
Authors |
Alexander Mathis, Jérôme Depaquit, Vit Dvořák, Holly Tuten, Anne-Laure Bañuls, Petr Halada, Sonia Zapata, Véronique Lehrter, Kristýna Hlavačková, Jorian Prudhomme, Petr Volf, Denis Sereno, Christian Kaufmann, Valentin Pflüger, Francis Schaffner |
Abstract |
Rapid, accurate and high-throughput identification of vector arthropods is of paramount importance in surveillance programmes that are becoming more common due to the changing geographic occurrence and extent of many arthropod-borne diseases. Protein profiling by MALDI-TOF mass spectrometry fulfils these requirements for identification, and reference databases have recently been established for several vector taxa, mostly with specimens from laboratory colonies. We established and validated a reference database containing 20 phlebotomine sand fly (Diptera: Psychodidae, Phlebotominae) species by using specimens from colonies or field-collections that had been stored for various periods of time. Identical biomarker mass patterns ('superspectra') were obtained with colony- or field-derived specimens of the same species. In the validation study, high quality spectra (i.e. more than 30 evaluable masses) were obtained with all fresh insects from colonies, and with 55/59 insects deep-frozen (liquid nitrogen/-80 °C) for up to 25 years. In contrast, only 36/52 specimens stored in ethanol could be identified. This resulted in an overall sensitivity of 87% (140/161); specificity was 100%. Duration of storage impaired data counts in the high mass range, and thus cluster analyses of closely related specimens might reflect their storage conditions rather than phenotypic distinctness. A major drawback of MALDI-TOF MS is the restricted availability of in-house databases and the fact that mass spectrometers from 2 companies (Bruker, Shimadzu) are widely being used. We have analysed fingerprints of phlebotomine sand flies obtained by automatic routine procedure on a Bruker instrument by using our database and the software established on a Shimadzu system. The sensitivity with 312 specimens from 8 sand fly species from laboratory colonies when evaluating only high quality spectra was 98.3%; the specificity was 100%. The corresponding diagnostic values with 55 field-collected specimens from 4 species were 94.7% and 97.4%, respectively. A centralized high-quality database (created by expert taxonomists and experienced users of mass spectrometers) that is easily amenable to customer-oriented identification services is a highly desirable resource. As shown in the present work, spectra obtained from different specimens with different instruments can be analysed using a centralized database, which should be available in the near future via an online platform in a cost-efficient manner. |
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