Title |
Pyrosequencing the transcriptome of the greenhouse whitefly, Trialeurodes vaporariorum reveals multiple transcripts encoding insecticide targets and detoxifying enzymes
|
---|---|
Published in |
BMC Genomics, January 2011
|
DOI | 10.1186/1471-2164-12-56 |
Pubmed ID | |
Authors |
Nikos Karatolos, Yannick Pauchet, Paul Wilkinson, Ritika Chauhan, Ian Denholm, Kevin Gorman, David R Nelson, Chris Bass, Richard H ffrench-Constant, Martin S Williamson |
Abstract |
The whitefly Trialeurodes vaporariorum is an economically important crop pest in temperate regions that has developed resistance to most classes of insecticides. However, the molecular mechanisms underlying resistance have not been characterised and, to date, progress has been hampered by a lack of nucleotide sequence data for this species. Here, we use pyrosequencing on the Roche 454-FLX platform to produce a substantial and annotated EST dataset. This 'unigene set' will form a critical reference point for quantitation of over-expressed messages via digital transcriptomics. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Germany | 1 | <1% |
Chile | 1 | <1% |
France | 1 | <1% |
Brazil | 1 | <1% |
United Kingdom | 1 | <1% |
Slovenia | 1 | <1% |
Mexico | 1 | <1% |
Greece | 1 | <1% |
Unknown | 115 | 93% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 31 | 25% |
Student > Ph. D. Student | 29 | 24% |
Student > Master | 20 | 16% |
Student > Doctoral Student | 6 | 5% |
Student > Postgraduate | 5 | 4% |
Other | 19 | 15% |
Unknown | 13 | 11% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 92 | 75% |
Biochemistry, Genetics and Molecular Biology | 5 | 4% |
Chemistry | 4 | 3% |
Nursing and Health Professions | 1 | <1% |
Linguistics | 1 | <1% |
Other | 5 | 4% |
Unknown | 15 | 12% |