Title |
Simple multiplex PCR assays to detect common pathogens and associated genes encoding for acquired extended spectrum betalactamases (ESBL) or carbapenemases from surgical site specimens in Vietnam
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Published in |
Annals of Clinical Microbiology and Antimicrobials, April 2015
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DOI | 10.1186/s12941-015-0079-z |
Pubmed ID | |
Authors |
Ngo Tat Trung, Tran Thi Thu Hien, Tran Thi Thanh Huyen, Dao Thanh Quyen, Mai Thanh Binh, Phan Quoc Hoan, Christian G Meyer, Thirumalaisamy P Velavan, Le Huu Song |
Abstract |
Surgical site infection (SSI) is common in Vietnamese post-operative patients. It contributes to increased morbidity, mortality, hospitalization time and health care expenditure. Bacterial culture is considered the gold standard procedure to identify SSI pathogens and antibiotic resistant properties; however, it can detect microbes that can readily grow and is time-consuming. We propose optimized multiplex PCR assays to diagnose the most relevant microbes and associated genes encoding for acquired extended spectrum betalactamases (ESBL) or carbapenemases from Vietnamese patients with SSI in a hospital setting in Hanoi. Ninety-one patients (n = 91) were collected in order to identify microbial pathogens and associated genes encoding for acquired extended spectrum betalactamases (ESBL) or carbapenemases by both conventional bacterial culture and in-house multiplex PCR assays. The novel in-house multiplex PCR assays are comparable to the bacterial culture approach in screening for common pathogens causing SSI and for relevant genotypes conferring betalactam/carbapenem resistance for bacteria. This is the first report of Turkey-specific ESBL gene (PER-1) and two Oxacilinase families (Oxa23 and Oxa 58) in Vietnam. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United Kingdom | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 80 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 16 | 20% |
Student > Ph. D. Student | 11 | 14% |
Researcher | 9 | 11% |
Student > Doctoral Student | 6 | 8% |
Student > Bachelor | 6 | 8% |
Other | 16 | 20% |
Unknown | 16 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 15 | 19% |
Immunology and Microbiology | 14 | 18% |
Biochemistry, Genetics and Molecular Biology | 14 | 18% |
Agricultural and Biological Sciences | 5 | 6% |
Nursing and Health Professions | 2 | 3% |
Other | 6 | 8% |
Unknown | 24 | 30% |