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Mendeley readers
Attention Score in Context
| Title |
Lipopolysaccharide preconditioning of adipose-derived stem cells improves liver-regenerating activity of the secretome
|
|---|---|
| Published in |
Stem Cell Research & Therapy, April 2015
|
| DOI | 10.1186/s13287-015-0072-7 |
| Pubmed ID | |
| Authors |
Sang Chul Lee, Hye Jin Jeong, Sang Kuon Lee, Say-June Kim |
| Abstract |
Growing recognition of paracrine mechanisms in stem cell plasticity has resulted in considerable interest in stem cell-derived secretome. The aim of this study was to investigate the effects of lipopolysaccharide (LPS) preconditioning on the composition and hepatic regenerative activity of adipose-derived stem cell (ASC) secretome. Conditioned medium (CM) and LPS-CM were obtained after culturing human ASCs without or with low-dose LPS (0.5 ng/ml) for 24 h, respectively. Untreated and thioacetamide-treated mouse AML12 hepatocytes were incubated for 24 h with the control medium, LPS (0.5 ng/ml), CM, and LPS-CM, respectively, and then cell viabilities were compared. CM and LPS-CM were also intravenously administrated to partially hepatectomized mice and their effects on liver regeneration were assessed using liver weight measurements, immunohistochemistry and western blotting. In the in vitro experiments, LPS preconditioning of ASCs enhanced the mRNA expression levels of IL-6, TNF-α, HGF, and VEGF, which evoke inflammatory response or liver regeneration. LPS-CM significantly promoted thioacetamide-damaged AML12 cell viability compared with CM-incubated cells and the control cells (77%, 69%, and 65%, respectively; P < 0.05). In the in vivo experiment, LPS-CM infusion into the partially hepatectomized mice significantly reduced serum IL-6 and TNF-α levels compared to the other groups (P < 0.05) on 1 and 2 days after partial hepatectomy. Moreover, LPS-CM infusion enhanced liver regeneration (based on the liver weight changes at 7 days after partial hepatectomy, 3.73% vs. 3.22% in the CM group; P < 0.05) and significantly reduced the elevated serum levels of AST and ALT (at day 1, P < 0.05). Our results suggest that LPS preconditioning effectively stimulates ASCs to produce the secretome beneficial to hepatic regeneration. Thus, optimizing ASC secretome profile by LPS preconditioning could be a promising approach to treat liver diseases using stem cells. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 55 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 55 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 12 | 22% |
| Researcher | 9 | 16% |
| Student > Bachelor | 5 | 9% |
| Student > Postgraduate | 5 | 9% |
| Student > Master | 5 | 9% |
| Other | 9 | 16% |
| Unknown | 10 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 11 | 20% |
| Medicine and Dentistry | 10 | 18% |
| Biochemistry, Genetics and Molecular Biology | 8 | 15% |
| Pharmacology, Toxicology and Pharmaceutical Science | 4 | 7% |
| Engineering | 3 | 5% |
| Other | 7 | 13% |
| Unknown | 12 | 22% |
Attention Score in Context
This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 June 2022.
All research outputs
#3,796,273
of 25,605,018 outputs
Outputs from Stem Cell Research & Therapy
#357
of 2,775 outputs
Outputs of similar age
#46,697
of 279,764 outputs
Outputs of similar age from Stem Cell Research & Therapy
#15
of 76 outputs
Altmetric has tracked 25,605,018 research outputs across all sources so far. Compared to these this one has done well and is in the 85th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,775 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.3. This one has done well, scoring higher than 86% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 279,764 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 83% of its contemporaries.
We're also able to compare this research output to 76 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 80% of its contemporaries.