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DNA methylation dynamics at imprinted genes during bovine pre-implantation embryo development

Overview of attention for article published in BMC Developmental Biology, March 2015
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Title
DNA methylation dynamics at imprinted genes during bovine pre-implantation embryo development
Published in
BMC Developmental Biology, March 2015
DOI 10.1186/s12861-015-0060-2
Pubmed ID
Authors

Alan M O’Doherty, David A Magee, Lynee C O’Shea, Niamh Forde, Marijke E Beltman, Solomon Mamo, Trudee Fair

Abstract

In mammals, maternal differentially methylated regions (DMRs) acquire DNA methylation during the postnatal growth stage of oogenesis, with paternal DMRs acquiring DNA methylation in the perinatal prospermatagonia. Following fusion of the male and female gametes, it is widely accepted that murine DNA methylation marks at the DMRs of imprinted genes are stable through embryogenesis and early development, until they are reprogrammed in primordial germ cells. However, the DNA methylation dynamics at DMRs of bovine imprinted genes during early stages of development remains largely unknown. The objective of this investigation was to analyse the methylation dynamics at imprinted gene DMRs during bovine embryo development, from blastocyst stage until implantation. To this end, pyrosequencing technology was used to quantify DNA methylation at DMR-associated CpG dinucleotides of six imprinted bovine genes (SNRPN, MEST, IGF2R, PLAGL1, PEG10 and H19) using bisulfite-modified genomic DNA isolated from individual blastocysts (Day 7); ovoid embryos (Day 14); filamentous embryos (Day 17) and implanting conceptuses (Day 25). For all genes, the degree of DNA methylation was most variable in Day 7 blastocysts compared to later developmental stages (P < 0.05). Furthermore, mining of RNA-seq transcriptomic data and western blot analysis revealed a specific window of expression of DNA methylation machinery genes (including DNMT3A, DNMT3B, TRIM28/KAP1 and DNMT1) and proteins (DNMT3A, DNMT3A2 and DNMT3B) by bovine embryos coincident with imprint stabilization. The findings of this study suggest that the DNA methylation status of bovine DMRs might be variable during the early stages of embryonic development, possibly requiring an active period of imprint stabilization.

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The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 56 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Spain 1 2%
India 1 2%
Canada 1 2%
Unknown 53 95%

Demographic breakdown

Readers by professional status Count As %
Student > Master 13 23%
Researcher 13 23%
Student > Ph. D. Student 10 18%
Student > Doctoral Student 5 9%
Student > Bachelor 4 7%
Other 4 7%
Unknown 7 13%
Readers by discipline Count As %
Agricultural and Biological Sciences 20 36%
Biochemistry, Genetics and Molecular Biology 12 21%
Psychology 4 7%
Veterinary Science and Veterinary Medicine 2 4%
Medicine and Dentistry 2 4%
Other 4 7%
Unknown 12 21%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 August 2016.
All research outputs
#10,647,130
of 16,638,522 outputs
Outputs from BMC Developmental Biology
#231
of 341 outputs
Outputs of similar age
#129,947
of 236,822 outputs
Outputs of similar age from BMC Developmental Biology
#1
of 1 outputs
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