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X Demographics
Mendeley readers
Attention Score in Context
| Title |
CLICK: one-step generation of conditional knockout mice
|
|---|---|
| Published in |
BMC Genomics, May 2018
|
| DOI | 10.1186/s12864-018-4713-y |
| Pubmed ID | |
| Authors |
Yoshiki Miyasaka, Yoshihiro Uno, Kazuto Yoshimi, Yayoi Kunihiro, Takuji Yoshimura, Tomohiro Tanaka, Harumi Ishikubo, Yuichi Hiraoka, Norihiko Takemoto, Takao Tanaka, Yoshihiro Ooguchi, Paul Skehel, Tomomi Aida, Junji Takeda, Tomoji Mashimo |
| Abstract |
CRISPR/Cas9 enables the targeting of genes in zygotes; however, efficient approaches to create loxP-flanked (floxed) alleles remain elusive. Here, we show that the electroporation of Cas9, two gRNAs, and long single-stranded DNA (lssDNA) into zygotes, termed CLICK (CRISPR with lssDNA inducing conditional knockout alleles), enables the quick generation of floxed alleles in mice and rats. The high efficiency of CLICK provides homozygous knock-ins in oocytes carrying tissue-specific Cre, which allows the one-step generation of conditional knockouts in founder (F0) mice. |
X Demographics
The data shown below were collected from the profiles of 13 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 3 | 23% |
| Spain | 2 | 15% |
| Mexico | 1 | 8% |
| United Kingdom | 1 | 8% |
| Unknown | 6 | 46% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 8 | 62% |
| Scientists | 3 | 23% |
| Practitioners (doctors, other healthcare professionals) | 1 | 8% |
| Science communicators (journalists, bloggers, editors) | 1 | 8% |
Mendeley readers
The data shown below were compiled from readership statistics for 149 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 149 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 39 | 26% |
| Student > Ph. D. Student | 17 | 11% |
| Student > Bachelor | 11 | 7% |
| Other | 10 | 7% |
| Student > Doctoral Student | 9 | 6% |
| Other | 27 | 18% |
| Unknown | 36 | 24% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 39 | 26% |
| Agricultural and Biological Sciences | 30 | 20% |
| Neuroscience | 14 | 9% |
| Medicine and Dentistry | 10 | 7% |
| Immunology and Microbiology | 4 | 3% |
| Other | 12 | 8% |
| Unknown | 40 | 27% |
Attention Score in Context
This research output has an Altmetric Attention Score of 13. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 October 2020.
All research outputs
#2,588,193
of 23,989,432 outputs
Outputs from BMC Genomics
#796
of 10,872 outputs
Outputs of similar age
#54,171
of 329,929 outputs
Outputs of similar age from BMC Genomics
#28
of 243 outputs
Altmetric has tracked 23,989,432 research outputs across all sources so far. Compared to these this one has done well and is in the 89th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 10,872 research outputs from this source. They receive a mean Attention Score of 4.8. This one has done particularly well, scoring higher than 92% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 329,929 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 83% of its contemporaries.
We're also able to compare this research output to 243 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 88% of its contemporaries.