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Small cell and non small cell lung cancer form metastasis on cellular 4D lung model

Overview of attention for article published in BMC Cancer, April 2018
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Title
Small cell and non small cell lung cancer form metastasis on cellular 4D lung model
Published in
BMC Cancer, April 2018
DOI 10.1186/s12885-018-4358-x
Pubmed ID
Authors

Dhruva K. Mishra, Ross A. Miller, Kristi A. Pence, Min P. Kim

Abstract

Metastasis is the main cause of death for lung cancer patients. The ex vivo 4D acellular lung model has been shown to mimic this metastatic process. However, the main concern is the model's lack of cellular components of the tumor's microenvironment. In this study, we aim to determine if the intact lung microenvironment will still allow lung cancer metastasis to form. We harvested a heart-lung block from a rat and placed it in a bioreactor after cannulating the pulmonary artery, trachea and tying the right main bronchus for 10-15 days without any tumor cells as a control group or with NSCLC (A549, H1299 or H460), SCLC (H69, H446 or SHP77) or breast cancer cell lines (MCF7 or MDAMB231) through the trachea. We performed lobectomy, H&E staining and IHC for human mitochondria to determine the primary tumor's growth and formation of metastatic lesions. In addition, we isolated circulating tumor cells (CTC) from the model seeded with GFP tagged cells. In the control group, no gross tumor nodules were found, H&E staining showed hyperplastic cells and IHC showed no staining for human mitochondria. All of the models seeded with cancer cell lines formed gross primary tumor nodules that had microscopic characteristics of human cancer cells on H&E staining with IHC showing staining for human mitochondria. CTC were isolated for those cells labeled with GFP and they were viable in culture. Finally, all cell lines formed metastatic lesions with cells stained for human mitochondria. The cellular ex vivo 4D model shows that human cancer cells can form a primary tumor, CTC and metastatic lesions in an intact cellular environment. This study suggests that the natural matrix scaffold is the only necessary component to drive metastatic progression and that cellular components play a role in modulating tumor progression.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 30 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 30 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 6 20%
Student > Master 5 17%
Student > Bachelor 5 17%
Researcher 4 13%
Student > Doctoral Student 2 7%
Other 1 3%
Unknown 7 23%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 12 40%
Pharmacology, Toxicology and Pharmaceutical Science 2 7%
Agricultural and Biological Sciences 2 7%
Medicine and Dentistry 2 7%
Chemistry 2 7%
Other 3 10%
Unknown 7 23%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 April 2018.
All research outputs
#12,201,867
of 13,779,708 outputs
Outputs from BMC Cancer
#4,191
of 5,172 outputs
Outputs of similar age
#234,358
of 270,489 outputs
Outputs of similar age from BMC Cancer
#1
of 1 outputs
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So far Altmetric has tracked 5,172 research outputs from this source. They receive a mean Attention Score of 4.1. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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