Title |
miR-1236-3p inhibits invasion and metastasis in gastric cancer by targeting MTA2
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Published in |
Cancer Cell International, May 2018
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DOI | 10.1186/s12935-018-0560-9 |
Pubmed ID | |
Authors |
Jia-Xiang An, Ming-Hui Ma, Chun-Dong Zhang, Shuai Shao, Nuo-Ming Zhou, Dong-Qiu Dai |
Abstract |
MicroRNAs deregulation are common in human tumor progression. miR-1236-3p has been reported to function as tumor suppressor microRNA in various malignancies. The aim of this study was to demonstrate the downregulated expression of miR-1236-3p in gastric cancer (GC) tissues and cell lines, and clarify its biological function in GC. Real-time polymerase chain reaction was used to measure the mRNA level of miR-1236-3p in GC. Dual luciferase assay was used to demonstrate that MTA2 was one of the candidate target genes of miR-1236-3p. Western blots were utilized to detect the protein levels. Cell function assays were also performed to determine the function of miR-1236-3p in GC. miR-1236-3p expression, which was associated with lymph node metastasis, differentiation and clinical stage, was significantly reduced in GC tissues and cell lines. miR-1236-3p over-expression could inhibit GC cell proliferation, migration and invasion, and inhibition of miR-1236-3p expression had opposite effects. Furthermore, we demonstrated that MTA2 was a candidate target of miR-1236-3p, and miR-1236-3p over-expression significantly inhibited the process of epithelial-mesenchymal transition. We also found that miR-1236-3p could suppress the PI3K/Akt signaling pathway in GC cells. Our results suggest that miR-1236-3p functions as a tumor suppressor in GC and could be a promising therapeutic target for GC. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 9 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 2 | 22% |
Unspecified | 1 | 11% |
Student > Bachelor | 1 | 11% |
Researcher | 1 | 11% |
Lecturer > Senior Lecturer | 1 | 11% |
Other | 0 | 0% |
Unknown | 3 | 33% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 2 | 22% |
Unspecified | 1 | 11% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 11% |
Agricultural and Biological Sciences | 1 | 11% |
Unknown | 4 | 44% |