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X Demographics
Mendeley readers
Attention Score in Context
| Title |
An improved method for Agrobacterium rhizogenes-mediated transformation of tomato suitable for the study of arbuscular mycorrhizal symbiosis
|
|---|---|
| Published in |
Plant Methods, May 2018
|
| DOI | 10.1186/s13007-018-0304-9 |
| Pubmed ID | |
| Authors |
Tania Ho-Plágaro, Raúl Huertas, María I. Tamayo-Navarrete, Juan A. Ocampo, José M. García-Garrido |
| Abstract |
Solanum lycopersicum, an economically important crop grown worldwide, has been used as a model for the study of arbuscular mycorrhizal (AM) symbiosis in non-legume plants for several years and several cDNA array hybridization studies have revealed specific transcriptomic profiles of mycorrhizal tomato roots. However, a method to easily screen candidate genes which could play an important role during tomato mycorrhization is required. We have developed an optimized procedure for composite tomato plant obtaining achieved through Agrobacterium rhizogenes-mediated transformation. This protocol involves the unusual in vitro culture of composite plants between two filter papers placed on the culture media. In addition, we show that DsRed is an appropriate molecular marker for the precise selection of cotransformed tomato hairy roots. S. lycopersicum composite plant hairy roots appear to be colonized by the AM fungus Rhizophagus irregularis in a manner similar to that of normal roots, and a modified construct useful for localizing the expression of promoters putatively associated with mycorrhization was developed and tested. In this study, we present an easy, fast and low-cost procedure to study AM symbiosis in tomato roots. |
X Demographics
The data shown below were collected from the profiles of 11 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Spain | 2 | 18% |
| Mexico | 1 | 9% |
| Japan | 1 | 9% |
| Indonesia | 1 | 9% |
| Zimbabwe | 1 | 9% |
| Canada | 1 | 9% |
| United Kingdom | 1 | 9% |
| Unknown | 3 | 27% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 6 | 55% |
| Scientists | 4 | 36% |
| Science communicators (journalists, bloggers, editors) | 1 | 9% |
Mendeley readers
The data shown below were compiled from readership statistics for 124 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 124 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 23 | 19% |
| Student > Master | 17 | 14% |
| Researcher | 16 | 13% |
| Student > Bachelor | 10 | 8% |
| Student > Doctoral Student | 5 | 4% |
| Other | 17 | 14% |
| Unknown | 36 | 29% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 41 | 33% |
| Biochemistry, Genetics and Molecular Biology | 25 | 20% |
| Immunology and Microbiology | 3 | 2% |
| Engineering | 2 | 2% |
| Environmental Science | 2 | 2% |
| Other | 7 | 6% |
| Unknown | 44 | 35% |
Attention Score in Context
This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 July 2020.
All research outputs
#5,556,372
of 23,047,237 outputs
Outputs from Plant Methods
#309
of 1,091 outputs
Outputs of similar age
#95,450
of 327,425 outputs
Outputs of similar age from Plant Methods
#11
of 25 outputs
Altmetric has tracked 23,047,237 research outputs across all sources so far. Compared to these this one has done well and is in the 75th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,091 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.3. This one has gotten more attention than average, scoring higher than 71% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 327,425 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 70% of its contemporaries.
We're also able to compare this research output to 25 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 56% of its contemporaries.