Title |
Scalable microfluidics for single-cell RNA printing and sequencing
|
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Published in |
Genome Biology, June 2015
|
DOI | 10.1186/s13059-015-0684-3 |
Pubmed ID | |
Authors |
Sayantan Bose, Zhenmao Wan, Ambrose Carr, Abbas H. Rizvi, Gregory Vieira, Dana Pe’er, Peter A. Sims |
Abstract |
Many important biological questions demand single-cell transcriptomics on a large scale. Hence, new tools are urgently needed for efficient, inexpensive manipulation of RNA from individual cells. We report a simple platform for trapping single-cell lysates in sealed, picoliter microwells capable of printing RNA on glass or capturing RNA on beads. We then develop a scalable technology for genome-wide, single-cell RNA-Seq. Our device generates pooled libraries from hundreds of individual cells with consumable costs of $0.10-$0.20 per cell and includes five lanes for simultaneous experiments. We anticipate that this system will serve as a general platform for single-cell imaging and sequencing. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 3 | 17% |
United Kingdom | 2 | 11% |
Germany | 1 | 6% |
New Zealand | 1 | 6% |
Denmark | 1 | 6% |
Australia | 1 | 6% |
Japan | 1 | 6% |
Canada | 1 | 6% |
Guinea | 1 | 6% |
Other | 2 | 11% |
Unknown | 4 | 22% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 9 | 50% |
Members of the public | 8 | 44% |
Science communicators (journalists, bloggers, editors) | 1 | 6% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 4 | 1% |
Sweden | 2 | <1% |
Korea, Republic of | 1 | <1% |
Norway | 1 | <1% |
Malaysia | 1 | <1% |
United Kingdom | 1 | <1% |
Czechia | 1 | <1% |
Denmark | 1 | <1% |
Slovenia | 1 | <1% |
Other | 0 | 0% |
Unknown | 258 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 63 | 23% |
Researcher | 61 | 23% |
Student > Bachelor | 25 | 9% |
Student > Master | 22 | 8% |
Other | 16 | 6% |
Other | 46 | 17% |
Unknown | 38 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 76 | 28% |
Biochemistry, Genetics and Molecular Biology | 68 | 25% |
Engineering | 23 | 8% |
Medicine and Dentistry | 10 | 4% |
Neuroscience | 8 | 3% |
Other | 40 | 15% |
Unknown | 46 | 17% |