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Scalable microfluidics for single-cell RNA printing and sequencing

Overview of attention for article published in Genome Biology, June 2015
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About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (95th percentile)
  • High Attention Score compared to outputs of the same age and source (82nd percentile)

Mentioned by

blogs
3 blogs
twitter
18 X users
patent
15 patents
facebook
1 Facebook page

Citations

dimensions_citation
113 Dimensions

Readers on

mendeley
271 Mendeley
citeulike
2 CiteULike
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Title
Scalable microfluidics for single-cell RNA printing and sequencing
Published in
Genome Biology, June 2015
DOI 10.1186/s13059-015-0684-3
Pubmed ID
Authors

Sayantan Bose, Zhenmao Wan, Ambrose Carr, Abbas H. Rizvi, Gregory Vieira, Dana Pe’er, Peter A. Sims

Abstract

Many important biological questions demand single-cell transcriptomics on a large scale. Hence, new tools are urgently needed for efficient, inexpensive manipulation of RNA from individual cells. We report a simple platform for trapping single-cell lysates in sealed, picoliter microwells capable of printing RNA on glass or capturing RNA on beads. We then develop a scalable technology for genome-wide, single-cell RNA-Seq. Our device generates pooled libraries from hundreds of individual cells with consumable costs of $0.10-$0.20 per cell and includes five lanes for simultaneous experiments. We anticipate that this system will serve as a general platform for single-cell imaging and sequencing.

X Demographics

X Demographics

The data shown below were collected from the profiles of 18 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 271 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 4 1%
Sweden 2 <1%
Korea, Republic of 1 <1%
Norway 1 <1%
Malaysia 1 <1%
United Kingdom 1 <1%
Czechia 1 <1%
Denmark 1 <1%
Slovenia 1 <1%
Other 0 0%
Unknown 258 95%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 63 23%
Researcher 61 23%
Student > Bachelor 25 9%
Student > Master 22 8%
Other 16 6%
Other 46 17%
Unknown 38 14%
Readers by discipline Count As %
Agricultural and Biological Sciences 76 28%
Biochemistry, Genetics and Molecular Biology 68 25%
Engineering 23 8%
Medicine and Dentistry 10 4%
Neuroscience 8 3%
Other 40 15%
Unknown 46 17%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 40. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 April 2024.
All research outputs
#1,033,404
of 25,613,746 outputs
Outputs from Genome Biology
#732
of 4,495 outputs
Outputs of similar age
#12,281
of 281,052 outputs
Outputs of similar age from Genome Biology
#12
of 64 outputs
Altmetric has tracked 25,613,746 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 95th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 4,495 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 27.6. This one has done well, scoring higher than 83% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 281,052 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 95% of its contemporaries.
We're also able to compare this research output to 64 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 82% of its contemporaries.