Title |
Polyelectrolyte-mediated increase of biofilm mass formation
|
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Published in |
BMC Microbiology, June 2015
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DOI | 10.1186/s12866-015-0457-x |
Pubmed ID | |
Authors |
Robert Bucki, Katarzyna Niemirowicz, Urszula Wnorowska, Marzena Wątek, Fitzroy J. Byfield, Katrina Cruz, Marta Wróblewska, Paul A. Janmey |
Abstract |
Biofilm formation is associated with various aspects of bacterial and fungal infection. This study was designed to assess the impact of diverse natural polyelectrolytes, such as DNA, F-actin, neurofilaments (NFs), vimentin and purified Pf1 bacteriophage on biofilm formation and swarming motility of select pathogens including Pseudomonas aeruginosa associated with lung infections in CF patients. The bacteriophage Pf1 (1 mg/ml) significantly increased biofilm mass produced by Pseudomonas aeruginosa P14, Escherichia coli RS218 and Bacillus subtilis ATCC6051. DNA, F-actin, NFs and Pf1 also increased biofilm mass of the fungal C. albicans 1409 strain. Addition of F-actin, DNA or Pf1 bacteriophage to 0.5% agar plates increased swarming motility of Pseudomonas aeruginosa Xen5. The presence of polyelectrolytes at infection sites is likely to promote biofilm growth and bacterial swarming. |
X Demographics
Geographical breakdown
Country | Count | As % |
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France | 1 | 33% |
Unknown | 2 | 67% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 67% |
Scientists | 1 | 33% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Portugal | 1 | 3% |
Unknown | 30 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 6 | 19% |
Other | 4 | 13% |
Student > Master | 3 | 10% |
Student > Doctoral Student | 3 | 10% |
Researcher | 3 | 10% |
Other | 2 | 6% |
Unknown | 10 | 32% |
Readers by discipline | Count | As % |
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Immunology and Microbiology | 6 | 19% |
Medicine and Dentistry | 5 | 16% |
Biochemistry, Genetics and Molecular Biology | 3 | 10% |
Agricultural and Biological Sciences | 3 | 10% |
Physics and Astronomy | 1 | 3% |
Other | 2 | 6% |
Unknown | 11 | 35% |