Title |
A combination of LongSAGE with Solexa sequencing is well suited to explore the depth and the complexity of transcriptome
|
---|---|
Published in |
BMC Genomics, September 2008
|
DOI | 10.1186/1471-2164-9-418 |
Pubmed ID | |
Authors |
Lucie Hanriot, Céline Keime, Nadine Gay, Claudine Faure, Carole Dossat, Patrick Wincker, Céline Scoté-Blachon, Christelle Peyron, Olivier Gandrillon |
Abstract |
"Open" transcriptome analysis methods allow to study gene expression without a priori knowledge of the transcript sequences. As of now, SAGE (Serial Analysis of Gene Expression), LongSAGE and MPSS (Massively Parallel Signature Sequencing) are the mostly used methods for "open" transcriptome analysis. Both LongSAGE and MPSS rely on the isolation of 21 pb tag sequences from each transcript. In contrast to LongSAGE, the high throughput sequencing method used in MPSS enables the rapid sequencing of very large libraries containing several millions of tags, allowing deep transcriptome analysis. However, a bias in the complexity of the transcriptome representation obtained by MPSS was recently uncovered. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 5 | 5% |
United Kingdom | 3 | 3% |
France | 2 | 2% |
Germany | 2 | 2% |
Brazil | 2 | 2% |
India | 1 | 1% |
Japan | 1 | 1% |
Norway | 1 | 1% |
Unknown | 78 | 82% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 37 | 39% |
Student > Ph. D. Student | 14 | 15% |
Student > Master | 10 | 11% |
Professor > Associate Professor | 9 | 9% |
Professor | 7 | 7% |
Other | 12 | 13% |
Unknown | 6 | 6% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 70 | 74% |
Biochemistry, Genetics and Molecular Biology | 8 | 8% |
Computer Science | 3 | 3% |
Engineering | 2 | 2% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 1% |
Other | 4 | 4% |
Unknown | 7 | 7% |