Title |
Next-generation DNA sequencing-based assay for measuring allelic expression imbalance (AEI) of candidate neuropsychiatric disorder genes in human brain
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Published in |
BMC Genomics, October 2011
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DOI | 10.1186/1471-2164-12-518 |
Pubmed ID | |
Authors |
Xiang Xu, Hao Wang, Min Zhu, Yue Sun, Yu Tao, Qin He, Jian Wang, Li Chen, David Saffen |
Abstract |
Common genetic variants that regulate gene expression are widely suspected to contribute to the etiology and phenotypic variability of complex diseases. Although high-throughput, microarray-based assays have been developed to measure differences in mRNA expression among independent samples, these assays often lack the sensitivity to detect rare mRNAs and the reproducibility to quantify small changes in mRNA expression. By contrast, PCR-based allelic expression imbalance (AEI) assays, which use a "marker" single nucleotide polymorphism (mSNP) in the mRNA to distinguish expression from pairs of genetic alleles in individual samples, have high sensitivity and accuracy, allowing differences in mRNA expression greater than 1.2-fold to be quantified with high reproducibility. In this paper, we describe the use of an efficient PCR/next-generation DNA sequencing-based assay to analyze allele-specific differences in mRNA expression for candidate neuropsychiatric disorder genes in human brain. |
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Members of the public | 3 | 100% |
Mendeley readers
Geographical breakdown
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United States | 1 | 2% |
France | 1 | 2% |
Unknown | 57 | 97% |
Demographic breakdown
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Researcher | 16 | 27% |
Student > Ph. D. Student | 14 | 24% |
Student > Master | 8 | 14% |
Professor > Associate Professor | 5 | 8% |
Professor | 4 | 7% |
Other | 6 | 10% |
Unknown | 6 | 10% |
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Mathematics | 3 | 5% |
Neuroscience | 3 | 5% |
Other | 7 | 12% |
Unknown | 9 | 15% |