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The Neisseria gonorrhoeae Obg protein is an essential ribosome-associated GTPase and a potential drug target

Overview of attention for article published in BMC Microbiology, June 2015
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Title
The Neisseria gonorrhoeae Obg protein is an essential ribosome-associated GTPase and a potential drug target
Published in
BMC Microbiology, June 2015
DOI 10.1186/s12866-015-0453-1
Pubmed ID
Authors

Ryszard A. Zielke, Igor H. Wierzbicki, Benjamin I. Baarda, Aleksandra E. Sikora

Abstract

Neisseria gonorrhoeae (GC) is a Gram-negative pathogen that most commonly infects mucosal surfaces, causing sexually transmitted urethritis in men and endocervicitis in women. Serious complications associated with these infections are frequent and include pelvic inflammatory disease, ectopic pregnancy, and infertility. The incidence of gonorrhea cases remains high globally while antibiotic treatment options, the sole counter measures against gonorrhea, are declining due to the remarkable ability of GC to acquire resistance. Evaluating of potential drug targets is essential to provide opportunities for developing antimicrobials with new mechanisms of action. We propose the GC Obg protein, belonging to the Obg/CgtA GTPase subfamily, as a potential target for the development of therapeutic interventions against gonorrhea, and in this study perform its initial functional and biochemical characterization. We report that NGO1990 encodes Obg protein, which is an essential factor for GC viability, associates predominantly with the large 50S ribosomal subunit, and is stably expressed under conditions relevant to infection of the human host. The anti-Obg antisera cross-reacts with a panel of contemporary GC clinical isolates, demonstrating the ubiquitous nature of Obg. The cellular levels of Obg reach a maximum in the early logarithmic phase and remain constant throughout bacterial growth. The in vitro binding and hydrolysis of the fluorescent guanine nucleotide analogs mant-GTP and mant-GDP by recombinant wild type and T192AT193A mutated variants of Obg are also assessed. Characterization of the GC Obg at the molecular and functional levels presented herein may facilitate the future targeting of this protein with small molecule inhibitors and the evaluation of identified lead compounds for bactericidal activity against GC and other drug-resistant bacteria.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 60 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 60 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 16 27%
Researcher 8 13%
Student > Bachelor 8 13%
Student > Master 5 8%
Lecturer 2 3%
Other 8 13%
Unknown 13 22%
Readers by discipline Count As %
Agricultural and Biological Sciences 14 23%
Biochemistry, Genetics and Molecular Biology 10 17%
Immunology and Microbiology 6 10%
Medicine and Dentistry 5 8%
Pharmacology, Toxicology and Pharmaceutical Science 3 5%
Other 8 13%
Unknown 14 23%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 July 2015.
All research outputs
#18,418,694
of 22,816,807 outputs
Outputs from BMC Microbiology
#2,242
of 3,188 outputs
Outputs of similar age
#188,899
of 262,924 outputs
Outputs of similar age from BMC Microbiology
#34
of 47 outputs
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We're also able to compare this research output to 47 others from the same source and published within six weeks on either side of this one. This one is in the 12th percentile – i.e., 12% of its contemporaries scored the same or lower than it.