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Quantitative analysis of an engineered CO2-fixing Escherichia coli reveals great potential of heterotrophic CO2 fixation

Overview of attention for article published in Biotechnology for Biofuels and Bioproducts, June 2015
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  • High Attention Score compared to outputs of the same age and source (87th percentile)

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1 blog

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123 Mendeley
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Title
Quantitative analysis of an engineered CO2-fixing Escherichia coli reveals great potential of heterotrophic CO2 fixation
Published in
Biotechnology for Biofuels and Bioproducts, June 2015
DOI 10.1186/s13068-015-0268-1
Pubmed ID
Authors

Fuyu Gong, Guoxia Liu, Xiaoyun Zhai, Jie Zhou, Zhen Cai, Yin Li

Abstract

Production of fuels from the abundant and wasteful CO2 is a promising approach to reduce carbon emission and consumption of fossil fuels. Autotrophic microbes naturally assimilate CO2 using energy from light, hydrogen, and/or sulfur. However, their slow growth rates call for investigation of the possibility of heterotrophic CO2 fixation. Although preliminary research has suggested that CO2 fixation in heterotrophic microbes is feasible after incorporation of a CO2-fixing bypass into the central carbon metabolic pathway, it remains unclear how much and how efficient that CO2 can be fixed by a heterotrophic microbe. A simple metabolic flux index was developed to indicate the relative strength of the CO2-fixation flux. When two sequential enzymes of the cyanobacterial Calvin cycle were incorporated into an E. coli strain, the flux of the CO2-fixing bypass pathway accounts for 13 % of that of the central carbon metabolic pathway. The value was increased to 17 % when the carbonic anhydrase involved in the cyanobacterial carbon concentrating mechanism was introduced, indicating that low intracellular CO2 concentration is one limiting factor for CO2 fixation in E. coli. The engineered CO2-fixing E. coli with carbonic anhydrase was able to fix CO2 at a rate of 19.6 mg CO2 L(-1) h(-1) or the specific rate of 22.5 mg CO2 g DCW(-1) h(-1). This CO2-fixation rate is comparable with the reported rates of 14 autotrophic cyanobacteria and algae (10.5-147.0 mg CO2 L(-1) h(-1) or the specific rates of 3.5-23.7 mg CO2 g DCW(-1) h(-1)). The ability of CO2 fixation was created and improved in E. coli by incorporating partial cyanobacterial Calvin cycle and carbon concentrating mechanism, respectively. Quantitative analysis revealed that the CO2-fixation rate of this strain is comparable with that of the autotrophic cyanobacteria and algae, demonstrating great potential of heterotrophic CO2 fixation.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 123 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 2 2%
Germany 1 <1%
India 1 <1%
Australia 1 <1%
Spain 1 <1%
United States 1 <1%
Unknown 116 94%

Demographic breakdown

Readers by professional status Count As %
Researcher 31 25%
Student > Ph. D. Student 28 23%
Student > Master 12 10%
Student > Bachelor 11 9%
Student > Doctoral Student 7 6%
Other 15 12%
Unknown 19 15%
Readers by discipline Count As %
Agricultural and Biological Sciences 38 31%
Biochemistry, Genetics and Molecular Biology 35 28%
Chemical Engineering 8 7%
Engineering 6 5%
Chemistry 5 4%
Other 7 6%
Unknown 24 20%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 29 June 2015.
All research outputs
#6,477,062
of 25,363,868 outputs
Outputs from Biotechnology for Biofuels and Bioproducts
#389
of 1,578 outputs
Outputs of similar age
#69,324
of 278,030 outputs
Outputs of similar age from Biotechnology for Biofuels and Bioproducts
#2
of 24 outputs
Altmetric has tracked 25,363,868 research outputs across all sources so far. This one has received more attention than most of these and is in the 74th percentile.
So far Altmetric has tracked 1,578 research outputs from this source. They receive a mean Attention Score of 4.9. This one has done well, scoring higher than 75% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 278,030 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 75% of its contemporaries.
We're also able to compare this research output to 24 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 87% of its contemporaries.