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Discovery of the combined oxidative cleavage of plant xylan and cellulose by a new fungal polysaccharide monooxygenase

Overview of attention for article published in Biotechnology for Biofuels, July 2015
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Title
Discovery of the combined oxidative cleavage of plant xylan and cellulose by a new fungal polysaccharide monooxygenase
Published in
Biotechnology for Biofuels, July 2015
DOI 10.1186/s13068-015-0284-1
Pubmed ID
Authors

Matthias Frommhagen, Stefano Sforza, Adrie H Westphal, Jaap Visser, Sandra W A Hinz, Martijn J Koetsier, Willem J H van Berkel, Harry Gruppen, Mirjam A Kabel

Abstract

Many agricultural and industrial food by-products are rich in cellulose and xylan. Their enzymatic degradation into monosaccharides is seen as a basis for the production of biofuels and bio-based chemicals. Lytic polysaccharide monooxygenases (LPMOs) constitute a group of recently discovered enzymes, classified as the auxiliary activity subgroups AA9, AA10, AA11 and AA13 in the CAZy database. LPMOs cleave cellulose, chitin, starch and β-(1 → 4)-linked substituted and non-substituted glucosyl units of hemicellulose under formation of oxidized gluco-oligosaccharides. Here, we demonstrate a new LPMO, obtained from Myceliophthora thermophila C1 (MtLPMO9A). This enzyme cleaves β-(1 → 4)-xylosyl bonds in xylan under formation of oxidized xylo-oligosaccharides, while it simultaneously cleaves β-(1 → 4)-glucosyl bonds in cellulose under formation of oxidized gluco-oligosaccharides. In particular, MtLPMO9A benefits from the strong interaction between low substituted linear xylan and cellulose. MtLPMO9A shows a strong synergistic effect with endoglucanase I (EGI) with a 16-fold higher release of detected oligosaccharides, compared to the oligosaccharides release of MtLPMO9A and EGI alone. Now, for the first time, we demonstrate the activity of a lytic polysaccharide monooxygenase (MtLPMO9A) that shows oxidative cleavage of xylan in addition to cellulose. The ability of MtLPMO9A to cleave these rigid regions provides a new paradigm in the understanding of the degradation of xylan-coated cellulose. In addition, MtLPMO9A acts in strong synergism with endoglucanase I. The mode of action of MtLPMO9A is considered to be important for loosening the rigid xylan-cellulose polysaccharide matrix in plant biomass, enabling increased accessibility to the matrix for hydrolytic enzymes. This discovery provides new insights into how to boost plant biomass degradation by enzyme cocktails for biorefinery applications.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 185 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 1 <1%
Finland 1 <1%
Thailand 1 <1%
Unknown 182 98%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 45 24%
Researcher 32 17%
Student > Master 27 15%
Student > Bachelor 20 11%
Student > Postgraduate 8 4%
Other 23 12%
Unknown 30 16%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 56 30%
Agricultural and Biological Sciences 49 26%
Chemistry 18 10%
Engineering 5 3%
Chemical Engineering 3 2%
Other 12 6%
Unknown 42 23%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 July 2015.
All research outputs
#4,464,983
of 5,364,474 outputs
Outputs from Biotechnology for Biofuels
#370
of 453 outputs
Outputs of similar age
#140,825
of 174,298 outputs
Outputs of similar age from Biotechnology for Biofuels
#15
of 16 outputs
Altmetric has tracked 5,364,474 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 453 research outputs from this source. They receive a mean Attention Score of 4.0. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 16 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.