Title |
Transcriptomic effects of di-(2-ethylhexyl)-phthalate in Syrian hamster embryo cells: an important role of early cytoskeleton disturbances in carcinogenesis?
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Published in |
BMC Genomics, October 2011
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DOI | 10.1186/1471-2164-12-524 |
Pubmed ID | |
Authors |
Yann Landkocz, Pascal Poupin, Franck Atienzar, Paule Vasseur |
Abstract |
Di-(2-ethylhexyl)-phthalate (DEHP) is a commonly used plasticizer in polyvinylchloride (PVC) formulations and a potentially non-genotoxic carcinogen. The aim of this study was to identify genes whose level of expression is altered by DEHP by using a global wide-genome approach in Syrian hamster embryo (SHE) cells, a model similar to human cells regarding their responses to this type of carcinogen. With mRNA Differential Display (DD), we analysed the transcriptional regulation of SHE cells exposed to 0, 12.5, 25 and 50 μM of DEHP for 24 hrs, conditions which induced neoplastic transformation of these cells. A real-time quantitative polymerase chain reaction (qPCR) was used to confirm differential expression of genes identified by DD. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 3 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 3 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Spain | 1 | 6% |
Russia | 1 | 6% |
Unknown | 16 | 89% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 7 | 39% |
Student > Postgraduate | 2 | 11% |
Professor > Associate Professor | 2 | 11% |
Student > Ph. D. Student | 2 | 11% |
Student > Doctoral Student | 1 | 6% |
Other | 3 | 17% |
Unknown | 1 | 6% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 7 | 39% |
Pharmacology, Toxicology and Pharmaceutical Science | 2 | 11% |
Biochemistry, Genetics and Molecular Biology | 2 | 11% |
Environmental Science | 1 | 6% |
Computer Science | 1 | 6% |
Other | 3 | 17% |
Unknown | 2 | 11% |