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Reconstruction of an SSR-based Magnaporthe oryzae physical map to locate avirulence gene AvrPi12

Overview of attention for article published in BMC Microbiology, May 2018
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Title
Reconstruction of an SSR-based Magnaporthe oryzae physical map to locate avirulence gene AvrPi12
Published in
BMC Microbiology, May 2018
DOI 10.1186/s12866-018-1192-x
Pubmed ID
Authors

Tonghui Li, Jianqiang Wen, Yaling Zhang, James Correll, Ling Wang, Qinghua Pan

Abstract

Pathogen avirulence (Avr) genes can evolve rapidly when challenged by the widespread deployment of host genes for resistance. They can be effectively isolated by positional cloning provided a robust and well-populated genetic map is available. An updated, SSR-based physical map of the rice blast pathogen Magnaporthe oryzae (Mo) has been constructed based on 116 of the 120 SSRs used to assemble the last map, along with 18 newly developed ones. A comparison between the two versions of the map has revealed an altered marker content and order within most of the Mo chromosomes. The avirulence gene AvrPi12 was mapped in a population of 219 progeny derived from a cross between the two Mo isolates CHL42 and CHL357. A bulked segregant analysis indicated that the gene was located on chromosome 6, a conclusion borne out by an analysis of the pattern of segregation shown by individual isolates. Six additional PCR-based markers were developed to improve the map resolution in the key region. AvrPi12 was finally located within the sub-telomeric region of chromosome 6, distal to the SSR locus LSM6-5. The improved SSR-based linkage map should be useful as a platform for gene mapping and isolation in Mo. It was used to establish the location of AvrPi12, thereby providing a starting point for its positional cloning.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 6 100%

Demographic breakdown

Readers by professional status Count As %
Unspecified 1 17%
Professor 1 17%
Student > Bachelor 1 17%
Student > Master 1 17%
Unknown 2 33%
Readers by discipline Count As %
Agricultural and Biological Sciences 2 33%
Unspecified 1 17%
Medicine and Dentistry 1 17%
Unknown 2 33%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 June 2018.
All research outputs
#17,974,941
of 23,083,773 outputs
Outputs from BMC Microbiology
#2,027
of 3,216 outputs
Outputs of similar age
#239,448
of 331,171 outputs
Outputs of similar age from BMC Microbiology
#20
of 42 outputs
Altmetric has tracked 23,083,773 research outputs across all sources so far. This one is in the 19th percentile – i.e., 19% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,216 research outputs from this source. They receive a mean Attention Score of 4.1. This one is in the 29th percentile – i.e., 29% of its peers scored the same or lower than it.
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We're also able to compare this research output to 42 others from the same source and published within six weeks on either side of this one. This one is in the 45th percentile – i.e., 45% of its contemporaries scored the same or lower than it.