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Effect of starvation on global gene expression and proteolysis in rainbow trout (Oncorhynchus mykiss)

Overview of attention for article published in BMC Genomics, September 2007
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About this Attention Score

  • Good Attention Score compared to outputs of the same age (66th percentile)
  • Good Attention Score compared to outputs of the same age and source (75th percentile)

Mentioned by

policy
1 policy source

Citations

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135 Dimensions

Readers on

mendeley
128 Mendeley
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1 CiteULike
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Title
Effect of starvation on global gene expression and proteolysis in rainbow trout (Oncorhynchus mykiss)
Published in
BMC Genomics, September 2007
DOI 10.1186/1471-2164-8-328
Pubmed ID
Authors

Mohamed Salem, Jeff Silverstein, Caird E Rexroad, Jianbo Yao

Abstract

Fast, efficiently growing animals have increased protein synthesis and/or reduced protein degradation relative to slow, inefficiently growing animals. Consequently, minimizing the energetic cost of protein turnover is a strategic goal for enhancing animal growth. Characterization of gene expression profiles associated with protein turnover would allow us to identify genes that could potentially be used as molecular biomarkers to select for germplasm with improved protein accretion. We evaluated changes in hepatic global gene expression in response to 3-week starvation in rainbow trout (Oncorhynchus mykiss). Microarray analysis revealed a coordinated, down-regulated expression of protein biosynthesis genes in starved fish. In addition, the expression of genes involved in lipid metabolism/transport, aerobic respiration, blood functions and immune response were decreased in response to starvation. However, the microarray approach did not show a significant increase of gene expression in protein catabolic pathways. Further studies, using real-time PCR and enzyme activity assays, were performed to investigate the expression of genes involved in the major proteolytic pathways including calpains, the multi-catalytic proteasome and cathepsins. Starvation reduced mRNA expression of the calpain inhibitor, calpastatin long isoform (CAST-L), with a subsequent increase in the calpain catalytic activity. In addition, starvation caused a slight but significant increase in 20S proteasome activity without affecting mRNA levels of the proteasome genes. Neither the mRNA levels nor the activities of cathepsin D and L were affected by starvation. These results suggest a significant role of calpain and 20S proteasome pathways in protein mobilization as a source of energy during fasting and a potential association of the CAST-L gene with fish protein accretion.

Mendeley readers

The data shown below were compiled from readership statistics for 128 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Colombia 2 2%
United States 2 2%
Austria 1 <1%
Germany 1 <1%
South Africa 1 <1%
India 1 <1%
Belgium 1 <1%
Sweden 1 <1%
Unknown 118 92%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 34 27%
Researcher 32 25%
Student > Master 20 16%
Professor 7 5%
Student > Bachelor 7 5%
Other 19 15%
Unknown 9 7%
Readers by discipline Count As %
Agricultural and Biological Sciences 83 65%
Biochemistry, Genetics and Molecular Biology 18 14%
Environmental Science 7 5%
Veterinary Science and Veterinary Medicine 4 3%
Arts and Humanities 1 <1%
Other 4 3%
Unknown 11 9%

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 October 2008.
All research outputs
#3,577,608
of 12,373,620 outputs
Outputs from BMC Genomics
#2,328
of 7,296 outputs
Outputs of similar age
#72,664
of 237,136 outputs
Outputs of similar age from BMC Genomics
#2
of 8 outputs
Altmetric has tracked 12,373,620 research outputs across all sources so far. This one is in the 49th percentile – i.e., 49% of other outputs scored the same or lower than it.
So far Altmetric has tracked 7,296 research outputs from this source. They receive a mean Attention Score of 4.3. This one has gotten more attention than average, scoring higher than 60% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 237,136 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 66% of its contemporaries.
We're also able to compare this research output to 8 others from the same source and published within six weeks on either side of this one. This one has scored higher than 6 of them.