Title |
Seroprevalence report on tick-borne encephalitis virus and Crimean-Congo hemorrhagic fever virus among Malaysian’s farm workers
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Published in |
BMC Public Health, July 2015
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DOI | 10.1186/s12889-015-1901-4 |
Pubmed ID | |
Authors |
Munirah Mohd Shukri, Kai Ling Kho, Masoumeh Ghane Kisomi, Rafidah Lani, Suria Marlina, Siti Fatimah Muhd Radzi, Sun Tee Tay, Li Ping Wong, Awang Bulgiba Awang Mahmud, Quaza Nizamuddin Hassan Nizam, Sazaly Abu Bakar, Keivan Zandi |
Abstract |
Tick-borne encephalitis virus (TBEV) and Crimean-Congo haemorrhagic fever virus (CCHFV) are important tick-borne viruses. Despite their wide geographical distribution and ease of acquisition, the prevalence of both viruses in Malaysia is still unknown. This study was conducted to determine the seroprevalence for TBEV and CCHFV among Malaysian farm workers as a high-risk group within the population. We gave questionnaires to 209 farm workers and invited them to participate in the study. Eighty-five agreed to do so. We then collected and tested sera for the presence of anti-TBEV IgG (immunoglobulin G) and anti-CCHFV IgG using a commercial enzyme-linked immunosorbent assay (ELISA) kit. We also tested seroreactive samples against three other related flaviviruses: dengue virus (DENV), West Nile virus (WNV) and Japanese encephalitis virus (JEV) using the ELISA method. The preliminary results showed the presence of anti-TBEV IgG in 31 (36.5 %) of 85 sera. However, when testing all the anti-TBEV IgG positive sera against the other three antigenically related flaviviruses to exclude possible cross reactivity, only five (4.2 %) sera did not show any cross reactivity. Interestingly, most (70.97 %) seropositives subjects mentioned tick-bite experience. However, there was no seroreactive sample for CCHFV. These viruses migrate to neighbouring countries so they should be considered threats for the future, despite the low seroprevalence for TBEV and no serological evidence for CCHFV in this study. Therefore, further investigation involving a large number of human, animal and tick samples that might reveal the viruses' true prevalence is highly recommended. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 98 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Unspecified | 18 | 18% |
Student > Ph. D. Student | 17 | 17% |
Researcher | 13 | 13% |
Student > Bachelor | 12 | 12% |
Student > Master | 12 | 12% |
Other | 16 | 16% |
Unknown | 10 | 10% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 25 | 26% |
Unspecified | 18 | 18% |
Immunology and Microbiology | 9 | 9% |
Biochemistry, Genetics and Molecular Biology | 6 | 6% |
Veterinary Science and Veterinary Medicine | 6 | 6% |
Other | 17 | 17% |
Unknown | 17 | 17% |