Title |
Deeply sequenced metagenome and metatranscriptome of a biogas-producing microbial community from an agricultural production-scale biogas plant
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Published in |
Giga Science, July 2015
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DOI | 10.1186/s13742-015-0073-6 |
Pubmed ID | |
Authors |
Andreas Bremges, Irena Maus, Peter Belmann, Felix Eikmeyer, Anika Winkler, Andreas Albersmeier, Alfred Pühler, Andreas Schlüter, Alexander Sczyrba |
Abstract |
The production of biogas takes place under anaerobic conditions and involves microbial decomposition of organic matter. Most of the participating microbes are still unknown and non-cultivable. Accordingly, shotgun metagenome sequencing currently is the method of choice to obtain insights into community composition and the genetic repertoire. Here, we report on the deeply sequenced metagenome and metatranscriptome of a complex biogas-producing microbial community from an agricultural production-scale biogas plant. We assembled the metagenome and, as an example application, show that we reconstructed most genes involved in the methane metabolism, a key pathway involving methanogenesis performed by methanogenic Archaea. This result indicates that there is sufficient sequencing coverage for most downstream analyses. Sequenced at least one order of magnitude deeper than previous studies, our metagenome data will enable new insights into community composition and the genetic potential of important community members. Moreover, mapping of transcripts to reconstructed genome sequences will enable the identification of active metabolic pathways in target organisms. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United Kingdom | 3 | 14% |
United States | 3 | 14% |
Germany | 2 | 9% |
Canada | 2 | 9% |
Spain | 1 | 5% |
Greece | 1 | 5% |
Finland | 1 | 5% |
Hong Kong | 1 | 5% |
Iceland | 1 | 5% |
Other | 0 | 0% |
Unknown | 7 | 32% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 12 | 55% |
Members of the public | 8 | 36% |
Science communicators (journalists, bloggers, editors) | 2 | 9% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Brazil | 5 | 3% |
United States | 3 | 2% |
Canada | 2 | 1% |
France | 1 | <1% |
Japan | 1 | <1% |
Germany | 1 | <1% |
Unknown | 160 | 92% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 43 | 25% |
Researcher | 32 | 18% |
Student > Master | 20 | 12% |
Student > Bachelor | 13 | 8% |
Student > Doctoral Student | 11 | 6% |
Other | 25 | 14% |
Unknown | 29 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 57 | 33% |
Biochemistry, Genetics and Molecular Biology | 30 | 17% |
Environmental Science | 17 | 10% |
Engineering | 10 | 6% |
Computer Science | 6 | 3% |
Other | 11 | 6% |
Unknown | 42 | 24% |