Title |
Comparative proteomics analysis of Spodoptera frugiperda cells during Autographa californica multiple nucleopolyhedrovirus infection
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Published in |
Virology Journal, August 2015
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DOI | 10.1186/s12985-015-0346-9 |
Pubmed ID | |
Authors |
Qian Yu, Youhua Xiong, Hang Gao, Jianliang Liu, Zhiqiang Chen, Qin Wang, Dongling Wen |
Abstract |
Increasing evidence sugggest that in addition of balculovirus controling insect host, host cells also responds to balculovirus infection. However, compared to existing knowledge on virus gene, host cell responses are relatively poorly understood. In this study, Spodoptera frugiperda (Sf9) cells were infected with Autographa californica multiple nucleopolyhedrovirus (AcMNPV). The protein composition and protein changes of Spodoptera frugiperda (Sf9) cells of different infection stages were analysed by isobaric tag for relative and absolute quantification (iTRAQ) techniques. A total of 4004 Sf9 proteins were identified by iTRAQ and 413 proteins were found as more than 1.5-fold changes in abundance. The 413 proteins were categorised according to GO classification for insects and were categorised into: biological process, molecular function and cellular component. The determination of the protein changes in infected Sf9 cells would help to better understanding of host cell responses and facilitate better design of this virus-host cell interaction in pest insect control and other related fields. |
X Demographics
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Unknown | 2 | 100% |
Demographic breakdown
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Members of the public | 1 | 50% |
Science communicators (journalists, bloggers, editors) | 1 | 50% |
Mendeley readers
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Japan | 1 | 2% |
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Unknown | 40 | 95% |
Demographic breakdown
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Student > Master | 6 | 14% |
Researcher | 6 | 14% |
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Student > Bachelor | 2 | 5% |
Other | 5 | 12% |
Unknown | 10 | 24% |
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Chemistry | 1 | 2% |
Other | 0 | 0% |
Unknown | 11 | 26% |