Title |
Stimulation of osteoblast differentiation with guided ultrasound waves
|
---|---|
Published in |
Journal of Therapeutic Ultrasound, August 2015
|
DOI | 10.1186/s40349-015-0034-7 |
Pubmed ID | |
Authors |
James Macione, Daniel Long, Sterling Nesbitt, Scott Wentzell, Hiroki Yokota, Vaibhav Pandit, Shiva Kotha |
Abstract |
Ultrasound induces mechanical vibration and heat, causing differentiation and proliferation in osteoblasts. All known in vitro evaluations of ultrasound are, however, performed with longitudinal ultrasound waves. We addressed a question: Do other forms of ultrasound waves, such as guided waves (longitudinal and guided flexural) transduced at a remote location, enhance differentiation of osteoblast cells? In this study, we employed guided Lamb waves that were induced in a borosilicate glass slide (cortical bone mimic). An average energy of 10-30 mW/cm(2) for 20 min per day was applied to MC3T3 osteoblast-like cells, which were placed 30-75 mm distant from the transducer. The result revealed that guided waves significantly stimulated the differentiation and mineralization of MC3T3 cells. In particular, guided waves elevated mRNA expression levels of bone formation-related genes such as alkaline phosphatase, osteopontin, osteocalcin, osteoprotegerin, and bone sialoprotein on days 8 and 16. In addition, the amount of mineralization found via Alizarin red staining was increased by 157 % (p = 0.034). The amount of mineralization was found to be independent of distance from the transducer (p = 0.967). We demonstrate herein that ultrasound in a form of guided Lamb waves is capable of inducing osteoblast differentiation in vitro, and it may enable the stimulation of osteoblasts in vivo over a distance from the site of ultrasound application. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Brazil | 1 | 7% |
Unknown | 14 | 93% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 5 | 33% |
Student > Bachelor | 3 | 20% |
Professor > Associate Professor | 2 | 13% |
Professor | 1 | 7% |
Other | 1 | 7% |
Other | 2 | 13% |
Unknown | 1 | 7% |
Readers by discipline | Count | As % |
---|---|---|
Engineering | 4 | 27% |
Biochemistry, Genetics and Molecular Biology | 3 | 20% |
Medicine and Dentistry | 3 | 20% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 7% |
Agricultural and Biological Sciences | 1 | 7% |
Other | 0 | 0% |
Unknown | 3 | 20% |