Title |
Rapid typing of foot-and-mouth disease serotype Asia 1 by reverse transcription loop-mediated isothermal amplification
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Published in |
Virology Journal, October 2011
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DOI | 10.1186/1743-422x-8-489 |
Pubmed ID | |
Authors |
Hao-tai Chen, Jie Zhang, Yong-sheng Liu, Xiang-tao Liu |
Abstract |
A reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) assay was rapidly used to detect serotype Asia 1 of foot-and-mouth disease virus (FMDV) within 45 min at 61°C. All FMDV serotype Asia 1 reference strains were positive by RT-LAMP, while other viruses such as FMDV serotypes O, C, A and classical swine fever virus, swine vesicular disease virus, porcine reproductive and respiratory syndrome virus and Japanese encephalitis virus remained negative. Furthermore, FMDV sreotype Asia 1 positive samples were able to detect by RT-LAMP assay. This RT-LAMP assay may be suitable particularly for diagnosis of FMDV serotype Asia 1 infection in field stations. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 2% |
Denmark | 1 | 2% |
South Africa | 1 | 2% |
Unknown | 47 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 15 | 30% |
Student > Ph. D. Student | 11 | 22% |
Student > Bachelor | 3 | 6% |
Professor | 2 | 4% |
Student > Doctoral Student | 2 | 4% |
Other | 8 | 16% |
Unknown | 9 | 18% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 16 | 32% |
Veterinary Science and Veterinary Medicine | 9 | 18% |
Biochemistry, Genetics and Molecular Biology | 5 | 10% |
Medicine and Dentistry | 4 | 8% |
Immunology and Microbiology | 2 | 4% |
Other | 5 | 10% |
Unknown | 9 | 18% |