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X Demographics
Mendeley readers
Attention Score in Context
| Title |
l-Methionine repressible promoters for tuneable gene expression in Trichoderma reesei
|
|---|---|
| Published in |
Microbial Cell Factories, August 2015
|
| DOI | 10.1186/s12934-015-0308-3 |
| Pubmed ID | |
| Authors |
Robert H. Bischof, Jennifer Horejs, Benjamin Metz, Christian Gamauf, Christian P Kubicek, Bernhard Seiboth |
| Abstract |
Trichoderma reesei is the main producer of lignocellulolytic enzymes that are required for plant biomass hydrolysis in the biorefinery industry. Although the molecular toolbox for T. reesei is already well developed, repressible promoters for strain engineering and functional genomics studies are still lacking. One such promoter that is widely employed for yeasts is that of the L-methionine repressible MET3 gene, encoding ATP sulphurylase. We show that the MET3 system can only be applied for T. reesei when the cellulase inducing carbon source lactose is used but not when wheat straw, a relevant lignocellulosic substrate for enzyme production, is employed. We therefore performed a transcriptomic screen for genes that are L-methionine repressible in a wheat straw culture. This analysis retrieved 50 differentially regulated genes of which 33 were downregulated. Among these, genes encoding transport proteins as well as iron containing DszA like monooxygenases and TauD like dioxygenases were strongly overrepresented. We show that the promoter region of one of these dioxygenases can be used for the strongly repressible expression of the Aspergillus niger sucA encoded extracellular invertase in T. reesei wheat straw cultures. This system is also portable to other carbon sources including D-glucose and glycerol as demonstrated by the repressible expression of the Escherichia coli lacZ encoded ß-galactosidase in T. reesei. We describe a novel, versatile set of promoters for T. reesei that can be used to drive recombinant gene expression in wheat straw cultures at different expression strengths and in an L-methionine repressible manner. The dioxygenase promoter that we studied in detail is furthermore compatible with different carbon sources and therefore applicable for manipulating protein production as well as functional genomics with T. reesei. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 33% |
| Unknown | 2 | 67% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 29 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | 7% |
| China | 1 | 3% |
| Unknown | 26 | 90% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 8 | 28% |
| Student > Ph. D. Student | 5 | 17% |
| Student > Master | 4 | 14% |
| Student > Doctoral Student | 3 | 10% |
| Other | 2 | 7% |
| Other | 3 | 10% |
| Unknown | 4 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 11 | 38% |
| Biochemistry, Genetics and Molecular Biology | 9 | 31% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 3% |
| Medicine and Dentistry | 1 | 3% |
| Chemistry | 1 | 3% |
| Other | 0 | 0% |
| Unknown | 6 | 21% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 May 2016.
All research outputs
#17,768,879
of 22,824,164 outputs
Outputs from Microbial Cell Factories
#1,123
of 1,599 outputs
Outputs of similar age
#178,334
of 264,379 outputs
Outputs of similar age from Microbial Cell Factories
#33
of 44 outputs
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So far Altmetric has tracked 1,599 research outputs from this source. They receive a mean Attention Score of 4.4. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
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