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Mendeley readers
Attention Score in Context
| Title |
Whole genome sequencing of enriched chloroplast DNA using the Illumina GAII platform
|
|---|---|
| Published in |
Plant Methods, September 2010
|
| DOI | 10.1186/1746-4811-6-22 |
| Pubmed ID | |
| Authors |
Robin A Atherton, Bennet J McComish, Lara D Shepherd, Lorraine A Berry, Nick W Albert, Peter J Lockhart |
| Abstract |
Complete chloroplast genome sequences provide a valuable source of molecular markers for studies in molecular ecology and evolution of plants. To obtain complete genome sequences, recent studies have made use of the polymerase chain reaction to amplify overlapping fragments from conserved gene loci. However, this approach is time consuming and can be more difficult to implement where gene organisation differs among plants. An alternative approach is to first isolate chloroplasts and then use the capacity of high-throughput sequencing to obtain complete genome sequences. We report our findings from studies of the latter approach, which used a simple chloroplast isolation procedure, multiply-primed rolling circle amplification of chloroplast DNA, Illumina Genome Analyzer II sequencing, and de novo assembly of paired-end sequence reads. |
Mendeley readers
The data shown below were compiled from readership statistics for 174 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Brazil | 7 | 4% |
| Germany | 2 | 1% |
| Indonesia | 2 | 1% |
| Pakistan | 2 | 1% |
| Switzerland | 1 | <1% |
| France | 1 | <1% |
| Hungary | 1 | <1% |
| Netherlands | 1 | <1% |
| Australia | 1 | <1% |
| Other | 3 | 2% |
| Unknown | 153 | 88% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 46 | 26% |
| Student > Ph. D. Student | 37 | 21% |
| Student > Master | 21 | 12% |
| Professor | 13 | 7% |
| Student > Bachelor | 12 | 7% |
| Other | 29 | 17% |
| Unknown | 16 | 9% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 121 | 70% |
| Biochemistry, Genetics and Molecular Biology | 18 | 10% |
| Chemistry | 3 | 2% |
| Social Sciences | 2 | 1% |
| Environmental Science | 2 | 1% |
| Other | 9 | 5% |
| Unknown | 19 | 11% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 November 2010.
All research outputs
#22,778,604
of 25,394,764 outputs
Outputs from Plant Methods
#1,233
of 1,265 outputs
Outputs of similar age
#102,573
of 107,983 outputs
Outputs of similar age from Plant Methods
#2
of 2 outputs
Altmetric has tracked 25,394,764 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,265 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.3. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 107,983 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 2 others from the same source and published within six weeks on either side of this one.