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Over-expressing Akt in T cells to resist tumor immunosuppression and increase anti-tumor activity

Overview of attention for article published in BMC Cancer, August 2015
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (86th percentile)

Mentioned by

twitter
2 tweeters
patent
14 patents
peer_reviews
1 peer review site

Citations

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11 Dimensions

Readers on

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29 Mendeley
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Title
Over-expressing Akt in T cells to resist tumor immunosuppression and increase anti-tumor activity
Published in
BMC Cancer, August 2015
DOI 10.1186/s12885-015-1611-4
Pubmed ID
Authors

Yanhong Wu, Zhenling Deng, Yishu Tang, Shuren Zhang, Yu-Qian Zhang

Abstract

Tumor employs various means to escape immunosurveillance and inhibit immune attack, and strategies have been developed to counteract the inhibitory signals. However, due to the complex suppressive mechanisms in the tumor microenvironment, blocking one or a few inhibitory signals has only limited effects on therapeutic efficacy. Instead of targeting tumor immunosuppression, we considered from another point of view, and hypothesized that manipulating T cells to make them resist any known or unknown suppressive mechanism may be more effective for cancer treatment. We used OT-1 cells transduced with retroviruses encoding Akt and human peripheral blood lymphocytes (PBLs) transduced with retroviruses encoding both Akt and a chimeric antigen receptor (CAR) specific for tumor antigen EpCAM to examine the effect of over-expressing Akt on tumor specific T cells in tumor environment. We show that Akt activity of T cells in the tumor environment was inhibited, and over-expressing Akt in OT-1 cells increased the cytokine production and cell proliferation in the presence of B16-OVA tumor cells. What's more, adoptive transfer of OT-1 cells over-expressing Akt inhibited B16-OVA tumor growth and prolonged mouse survival. To examine if over-expressing Akt could increase the anti-tumor activity of T cells in human cancer, PBLs co-expressing EpCAM specific CAR and Akt were cultured with EpCAM-expressing human prostate cancer cells PC3M, and less inhibition on cell proliferation and less apoptosis were observed. In addition, adoptive transfer of PC3M specific T cells over-expressing Akt resulted in more dramatic tumor inhibitory effects in PC3M bearing NOD/SCID mice. These data indicates that over-expressing Akt in tumor specific T cells increases T cell proliferation and activity in the tumor environment, and enhances anti-tumor effects of adoptively transferred T cells. Our study provides a new strategy to improve the efficacy of adoptive T cell therapy, and serves as an important foundation for clinical translation.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 29 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Netherlands 1 3%
Unknown 28 97%

Demographic breakdown

Readers by professional status Count As %
Student > Master 6 21%
Student > Ph. D. Student 5 17%
Researcher 4 14%
Student > Bachelor 3 10%
Professor 2 7%
Other 3 10%
Unknown 6 21%
Readers by discipline Count As %
Agricultural and Biological Sciences 9 31%
Medicine and Dentistry 7 24%
Immunology and Microbiology 3 10%
Computer Science 1 3%
Psychology 1 3%
Other 1 3%
Unknown 7 24%

Attention Score in Context

This research output has an Altmetric Attention Score of 12. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 October 2022.
All research outputs
#2,564,131
of 22,545,203 outputs
Outputs from BMC Cancer
#504
of 8,197 outputs
Outputs of similar age
#34,101
of 255,256 outputs
Outputs of similar age from BMC Cancer
#1
of 1 outputs
Altmetric has tracked 22,545,203 research outputs across all sources so far. Compared to these this one has done well and is in the 88th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 8,197 research outputs from this source. They receive a mean Attention Score of 4.3. This one has done particularly well, scoring higher than 93% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 255,256 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 86% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them