Title |
In vivo rescue of alveolar macrophages from SP-A knockout mice with exogenous SP-A nearly restores a wild type intracellular proteome; actin involvement
|
---|---|
Published in |
Proteome Science, October 2011
|
DOI | 10.1186/1477-5956-9-67 |
Pubmed ID | |
Authors |
David S Phelps, Todd M Umstead, Omar A Quintero, Christopher M Yengo, Joanna Floros |
Abstract |
Mice lacking surfactant protein-A (SP-A-/-; knockout; KO) exhibit increased vulnerability to infection and injury. Although many bronchoalveolar lavage (BAL) protein differences between KO and wild-type (WT) are rapidly reversed in KO after infection, their clinical course is still compromised. We studied the impact of SP-A on the alveolar macrophage (AM) proteome under basal conditions. Male SP-A KO mice were SP-A-treated (5 micrograms/mouse) and sacrificed in 6 or 18 hr. The AM proteomes of KO, SP-A-treated KO, and WT mice were studied by 2D-DIGE coupled with MALDI-ToF/ToF and AM actin distribution was examined by phalloidon staining. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
India | 1 | 5% |
France | 1 | 5% |
Unknown | 20 | 91% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 8 | 36% |
Student > Master | 3 | 14% |
Professor | 2 | 9% |
Professor > Associate Professor | 2 | 9% |
Other | 1 | 5% |
Other | 2 | 9% |
Unknown | 4 | 18% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 6 | 27% |
Agricultural and Biological Sciences | 5 | 23% |
Biochemistry, Genetics and Molecular Biology | 2 | 9% |
Unspecified | 1 | 5% |
Mathematics | 1 | 5% |
Other | 2 | 9% |
Unknown | 5 | 23% |