Title |
Carbapenem-resistant and carbapenem-susceptible isogenic isolates of Klebsiella pneumoniae ST101 causing infection in a tertiary hospital
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Published in |
BMC Microbiology, September 2015
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DOI | 10.1186/s12866-015-0510-9 |
Pubmed ID | |
Authors |
Meritxell Cubero, Guillermo Cuervo, M. Ángeles Dominguez, Fe Tubau, Sara Martí, Elena Sevillano, Lucía Gallego, Josefina Ayats, Carmen Peña, Miquel Pujol, Josefina Liñares, Carmen Ardanuy |
Abstract |
In this study we describe the clinical and molecular characteristics of an outbreak due to carbapenem-resistant Klebsiella pneumoniae (CR-KP) producing CTX-M-15 and OXA-48 carbapenemase. Isogenic strains, carbapenem-susceptible K. pneumoniae (CS-KP) producing CTX-M-15, were also involved in the outbreak. From October 2010 to December 2012 a total of 62 CR-KP and 23 CS-KP were isolated from clinical samples of 42 patients (22 had resistant isolates, 14 had susceptible isolates, and 6 had both CR and CS isolates). All patients had underlying diseases and 17 of them (14 patients with CR-KP and 3 with CS-KP) had received carbapenems previously. The range of carbapenem MICs for total isolates were: imipenem: 2 to >32 μg/ml vs. <2 μg/ml; meropenem: 4 to >32 μg/ml vs. <2 μg/ml; and ertapenem: 8 to >32 μg/ml vs. <2 μg/ml. All the isolates were also resistant to gentamicin, ciprofloxacin, and cotrimoxazole. Both types of isolates shared a common PFGE pattern associated with the multilocus sequence type 101 (ST101). The bla CTX-M-15 gene was detected in all the isolates, whereas the bla OXA-48 gene was only detected in CR-KP isolates on a 70 kb plasmid. The clonal spread of K. pneumoniae ST101 expressing the OXA-48 and CTX-M-15 beta-lactamases was the cause of an outbreak of CR-KP infections. CTX-M-15-producing isolates lacking the bla OXA-48 gene coexisted during the outbreak. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Spain | 1 | 17% |
United Kingdom | 1 | 17% |
Australia | 1 | 17% |
Unknown | 3 | 50% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 5 | 83% |
Practitioners (doctors, other healthcare professionals) | 1 | 17% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 73 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 16 | 22% |
Researcher | 15 | 21% |
Student > Master | 12 | 16% |
Student > Bachelor | 5 | 7% |
Student > Postgraduate | 4 | 5% |
Other | 11 | 15% |
Unknown | 10 | 14% |
Readers by discipline | Count | As % |
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Medicine and Dentistry | 16 | 22% |
Immunology and Microbiology | 14 | 19% |
Agricultural and Biological Sciences | 10 | 14% |
Biochemistry, Genetics and Molecular Biology | 7 | 10% |
Social Sciences | 3 | 4% |
Other | 3 | 4% |
Unknown | 20 | 27% |