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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Widespread Expression of Erythropoietin Receptor in Brain and Its Induction by Injury
|
|---|---|
| Published in |
Molecular Medicine, September 2015
|
| DOI | 10.2119/molmed.2015.00192 |
| Pubmed ID | |
| Authors |
Christoph Ott, Henrik Martens, Imam Hassouna, Barbara Oliveira, Christian Erck, Maria-Patapia Zafeiriou, Ulla-Kaisa Peteri, Dörte Hesse, Simone Gerhart, Bekir Altas, Tekla Kolbow, Herbert Stadler, Hiroshi Kawabe, Wolfram-Hubertus Zimmermann, Klaus-Armin Nave, Walter Schulz-Schaeffer, Olaf Jahn, Hannelore Ehrenreich |
| Abstract |
Erythropoietin (EPO) exerts potent neuroprotective, neuroregenerative and procognitive functions. However, unequivocal demonstration of erythropoietin receptor (EPOR) expression in brain cells has remained difficult since previously available anti-EPOR antibodies (EPOR-AB) were unspecific. We report here a new, highly specific, polyclonal rabbit EPOR-AB directed against different epitopes in the cytoplasmic tail of human and murine EPOR and its characterization by mass spectrometric analysis of immunoprecipitated endogenous EPOR, Western blotting, immunostaining, and flow cytometry. Among others, we applied genetic strategies including overexpression, Lentivirus-mediated conditional knockout of EpoR, and tagged proteins, both on cultured cells and tissue sections, as well as intracortical implantation of EPOR-transduced cells to verify specificity. We show examples of EPOR expression in neurons, oligodendroglia, astrocytes, and microglia. Employing this new EPOR-AB with double-labelling strategies, we demonstrate membrane expression of EPOR as well as its localization in intracellular compartments such as the Golgi apparatus. Moreover, we show injury-induced expression of EPOR: In mice, a stereotactically applied stab wound to the motor cortex leads to distinct EpoR expression by reactive GFAP-expressing cells in the lesion vicinity. In a patient suffering from epilepsy, neurons and oligodendrocytes of the hippocampus strongly express EPOR. To conclude, this new analytical tool will allow neuroscientists to pinpoint EPOR expression in cells of the nervous system and to better understand its role in healthy conditions, including brain development, as well as under pathological circumstances, such as upregulation upon distress and injury. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 3 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 65 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | 2% |
| Unknown | 64 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 12 | 18% |
| Student > Master | 10 | 15% |
| Student > Bachelor | 9 | 14% |
| Researcher | 7 | 11% |
| Student > Doctoral Student | 6 | 9% |
| Other | 8 | 12% |
| Unknown | 13 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Neuroscience | 14 | 22% |
| Medicine and Dentistry | 9 | 14% |
| Agricultural and Biological Sciences | 9 | 14% |
| Biochemistry, Genetics and Molecular Biology | 8 | 12% |
| Veterinary Science and Veterinary Medicine | 3 | 5% |
| Other | 7 | 11% |
| Unknown | 15 | 23% |
Attention Score in Context
This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 November 2023.
All research outputs
#7,171,149
of 25,337,969 outputs
Outputs from Molecular Medicine
#344
of 1,267 outputs
Outputs of similar age
#77,417
of 273,653 outputs
Outputs of similar age from Molecular Medicine
#2
of 11 outputs
Altmetric has tracked 25,337,969 research outputs across all sources so far. This one has received more attention than most of these and is in the 71st percentile.
So far Altmetric has tracked 1,267 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 8.2. This one has gotten more attention than average, scoring higher than 72% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 273,653 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 71% of its contemporaries.
We're also able to compare this research output to 11 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 90% of its contemporaries.