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Effects of freezing storage on the DNA extraction and microbial evaluation from anaerobic digested sludges

Overview of attention for article published in BMC Research Notes, September 2015
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  • Above-average Attention Score compared to outputs of the same age (60th percentile)

Mentioned by

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7 tweeters

Citations

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6 Dimensions

Readers on

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50 Mendeley
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Title
Effects of freezing storage on the DNA extraction and microbial evaluation from anaerobic digested sludges
Published in
BMC Research Notes, September 2015
DOI 10.1186/s13104-015-1407-2
Pubmed ID
Authors

Valeria Romanazzi, Deborah Traversi, Eugenio Lorenzi, Giorgio Gilli

Abstract

The anaerobic digestion is one of the most spread renewable energy technology. The input biomasses included various environmental problematic wastes such as sludge coming from wastewater treatment plant (WWTP) and organic fraction of municipal solid waste (OFMSW). As biomolecular procedures have become important tools for the microbial characterisation of anaerobic samples coming from the reactors, it is crucial sampling and extracting properly DNA in order to employ such types of techniques. The current study is aimed to evaluate how freezing temperature and length of storage at -20 °C influence both the extracted DNA yield and microbial community quantifications from digested sludge samples collected at full-scale plants. From WWTP sludge samples, we observed a reduction of DNA concentration comparing fresh and stored samples for 10 days at -20 °C (ANOVA test p < 0.0001), with an estimated DNA loss of approximately 65 % for such types of samples, however the methanogen communities can be assessed respecting the fresh conditions. From OFMSW sludge samples, we observed a reduction in extracted DNA (-90 %), after 120 frozen days, while microbial communities are determined respecting the fresh conditions within 2 months of frozen storage. The remarkable effect of frozen storage on sludge samples suggests as the better procedure to perform the DNA extraction from fresh sample. On the other hand it is not generally possible, so approximately 2 months of storage at -20 °C appears to be suitable time at which DNA concentrations remain sufficient to perform coherent microbial characterization through quantitative qRT-PCR.

Twitter Demographics

The data shown below were collected from the profiles of 7 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 50 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 50 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 13 26%
Student > Bachelor 11 22%
Student > Master 10 20%
Researcher 4 8%
Student > Doctoral Student 4 8%
Other 3 6%
Unknown 5 10%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 9 18%
Environmental Science 9 18%
Engineering 7 14%
Agricultural and Biological Sciences 7 14%
Medicine and Dentistry 3 6%
Other 7 14%
Unknown 8 16%

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 June 2016.
All research outputs
#6,268,283
of 19,293,994 outputs
Outputs from BMC Research Notes
#1,082
of 3,898 outputs
Outputs of similar age
#82,626
of 250,251 outputs
Outputs of similar age from BMC Research Notes
#1
of 1 outputs
Altmetric has tracked 19,293,994 research outputs across all sources so far. This one is in the 46th percentile – i.e., 46% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,898 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.1. This one has gotten more attention than average, scoring higher than 67% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 250,251 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 60% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them