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Saliva as an alternative specimen for detection of Schmallenberg virus-specific antibodies in bovines

Overview of attention for article published in BMC Veterinary Research, September 2015
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Title
Saliva as an alternative specimen for detection of Schmallenberg virus-specific antibodies in bovines
Published in
BMC Veterinary Research, September 2015
DOI 10.1186/s12917-015-0552-0
Pubmed ID
Authors

Justas Lazutka, Aliona Spakova, Vilimas Sereika, Raimundas Lelesius, Kestutis Sasnauskas, Rasa Petraityte-Burneikiene

Abstract

Schmallenberg virus (SBV), discovered in continental Europe in late 2011, causes mild clinical signs in adult ruminants, including diarrhoea and reduced milk yield. However, fetal infection can lead to severe malformation in newborn offspring. Enzyme-linked immunosorbent assays (ELISA) are commercially available for detection of SBV-specific antibodies in bovine sera and milk. Here we describe the development and evaluation of an indirect ELISA based on a yeast derived recombinant SBV nucleocapsid protein (N) for the detection of SBV-specific antibodies in bovine saliva. Development of a non-invasive test to detect antibodies in individual bovine saliva samples could potentially provide a test suitable for calves and adult cattle. The aim of this study was to investigate the agreement between the levels of antibodies (IgG) measured in milk and sera, and the level of antibodies (IgG and IgA) in saliva, in comparison with the antibody levels detected in sera and milk with commercially available test. Serum, milk and saliva samples from 58 cows were collected from three dairy herds in Lithuania and tested for the presence of SBV-specific antibodies. The presence of IgG antibodies was tested in parallel serum and milk samples, while the presence of IgA and IgG antibodies was tested in saliva samples. The presence of SBV-specific IgG and IgA in saliva was tested using an indirect ELISA based on a yeast-derived recombinant N protein. The presence of SBV-specific IgG in milk and sera was tested in parallel using a commercial recombinant protein based test. The sensitivities of the newly developed tests were as follows: 96 % for the IgG serum assay and 94 % for the IgG milk assay and 85 % and 98 % for IgG and IgA in saliva tests, when compared with data generated by a commercial IgG assay. Data from testing the saliva IgG and IgA and also the milk and serum IgG with indirect SBV-specific ELISAs showed close agreement with the commercial serum and milk IgG assay data. The level of IgG in saliva was notably lower in comparison to IgA. The newly developed method exhibits the potential to serve as an easily transferable tool for epidemiological studies.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 30 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 30 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 5 17%
Student > Master 5 17%
Researcher 4 13%
Student > Doctoral Student 3 10%
Student > Bachelor 3 10%
Other 3 10%
Unknown 7 23%
Readers by discipline Count As %
Agricultural and Biological Sciences 10 33%
Veterinary Science and Veterinary Medicine 8 27%
Medicine and Dentistry 4 13%
Immunology and Microbiology 1 3%
Nursing and Health Professions 1 3%
Other 0 0%
Unknown 6 20%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 September 2015.
All research outputs
#5,412,824
of 6,354,076 outputs
Outputs from BMC Veterinary Research
#802
of 990 outputs
Outputs of similar age
#147,962
of 184,868 outputs
Outputs of similar age from BMC Veterinary Research
#61
of 78 outputs
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