RT @RJSOrr77: @Thomieh @nanopore Hi @Thomieh, I have done this with pacbio but same problem, long product + PCR = chimeras https://t.co/0F0…
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@Thomieh @nanopore Hi @Thomieh, I have done this with pacbio but same problem, long product + PCR = chimeras https://t.co/0F0tfHjlUQ. Reduce PCR to an absolute minimum and use Usearch to remove chimeras. can always compare to reference seqeunces as well.
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RT @OlgaVPettersson: Sequencing near-complete 18S amplicons with PacBio: "...In agreement with previous studies, we find that long-read Pac…
1,700 followers
RT @OlgaVPettersson: Sequencing near-complete 18S amplicons with PacBio: "...In agreement with previous studies, we find that long-read Pac…