Title |
Challenge in direct Spoligotyping of Mycobacterium tuberculosis: a problematic issue in the region with high prevalence of polyclonal infections
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Published in |
BMC Research Notes, July 2018
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DOI | 10.1186/s13104-018-3579-z |
Pubmed ID | |
Authors |
Mansour Kargarpour Kamakoli, Sharareh Khanipour, Shima Hadifar, Hasan Ghajavand, Ghazaleh Farmanfarmaei, Abolfazl Fateh, Seyed Davar Siadat, Farzam Vaziri |
Abstract |
Based on our recent studies the prevalence of polyclonal infection in tuberculosis clinical specimens is more than 50% in Tehran, Iran. With this background, Spoligotyping was performed on clinical specimens and their respective cultures, and we examined whether mixed infections interfere with the results or not. Based on the Spoligotyping pattern, among the fourteen patients, 57.1% had different genotypes in clinical samples and their respective cultures. These discrepant patterns were suggestive of polyclonal infections in clinical samples with possible overlapping Spoligotype patterns. We propose that in societies with high mixed infections (e.g. Iran), direct Spoligotyping on clinical samples can be controversial. |
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United Kingdom | 1 | 100% |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 25 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 5 | 20% |
Student > Master | 4 | 16% |
Other | 2 | 8% |
Professor | 2 | 8% |
Student > Bachelor | 1 | 4% |
Other | 6 | 24% |
Unknown | 5 | 20% |
Readers by discipline | Count | As % |
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Medicine and Dentistry | 6 | 24% |
Agricultural and Biological Sciences | 4 | 16% |
Immunology and Microbiology | 4 | 16% |
Nursing and Health Professions | 1 | 4% |
Veterinary Science and Veterinary Medicine | 1 | 4% |
Other | 2 | 8% |
Unknown | 7 | 28% |