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A Streptomyces coelicolor host for the heterologous expression of Type III polyketide synthase genes

Overview of attention for article published in Microbial Cell Factories, September 2015
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3 tweeters

Citations

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Title
A Streptomyces coelicolor host for the heterologous expression of Type III polyketide synthase genes
Published in
Microbial Cell Factories, September 2015
DOI 10.1186/s12934-015-0335-0
Pubmed ID
Authors

Anyarat Thanapipatsiri, Jan Claesen, Juan-Pablo Gomez-Escribano, Mervyn Bibb, Arinthip Thamchaipenet

Abstract

Recent advances in genome sequencing, combined with bioinformatic analysis, has led to the identification of numerous novel natural product gene clusters, particularly in actinomycetes of terrestrial and marine origin. Many of these gene clusters encode uncharacterised Type III polyketide synthases. To facilitate the study of these genes and their potentially novel products, we set out to construct an actinomycete expression host specifically designed for the heterologous expression of Type III PKS genes and their gene clusters. A derivative of Streptomyces coelicolor A3(2) designed for the expression of Type III polyketide synthase (PKS) genes was constructed from the previously engineered expression strain S. coelicolor M1152 [Δact Δred Δcpk Δcda rpoB(C1298T)] by removal of all three of the endogenous Type III PKS genes (gcs, srsA, rppA) by PCR targeting. The resulting septuple deletion mutant, M1317, proved to be an effective surrogate host for the expression of actinobacterial Type III PKS genes: expression of the reintroduced gcs gene from S. coelicolor and of the heterologous rppA gene from Streptomyces venezuelae under the control of the constitutive ermE* promoter resulted in copious production of germicidin and flaviolin, respectively. The newly constructed expression host S. coelicolor M1317 should be particularly useful for the discovery and analysis of new Type III polyketide metabolites.

Twitter Demographics

The data shown below were collected from the profiles of 3 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 81 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
China 1 1%
Unknown 80 99%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 17 21%
Student > Master 14 17%
Researcher 13 16%
Student > Bachelor 7 9%
Other 4 5%
Other 15 19%
Unknown 11 14%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 29 36%
Agricultural and Biological Sciences 20 25%
Chemistry 8 10%
Pharmacology, Toxicology and Pharmaceutical Science 4 5%
Immunology and Microbiology 3 4%
Other 5 6%
Unknown 12 15%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 September 2015.
All research outputs
#3,000,030
of 6,364,199 outputs
Outputs from Microbial Cell Factories
#197
of 447 outputs
Outputs of similar age
#107,364
of 198,837 outputs
Outputs of similar age from Microbial Cell Factories
#11
of 35 outputs
Altmetric has tracked 6,364,199 research outputs across all sources so far. This one is in the 29th percentile – i.e., 29% of other outputs scored the same or lower than it.
So far Altmetric has tracked 447 research outputs from this source. They receive a mean Attention Score of 2.7. This one is in the 42nd percentile – i.e., 42% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 198,837 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 35th percentile – i.e., 35% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 35 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 57% of its contemporaries.