Title |
Advancing understanding of microbial bioenergy conversion processes by activity-based protein profiling
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Published in |
Biotechnology for Biofuels and Bioproducts, September 2015
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DOI | 10.1186/s13068-015-0343-7 |
Pubmed ID | |
Authors |
Yun Liu, James K. Fredrickson, Natalie C. Sadler, Premchendar Nandhikonda, Richard D. Smith, Aaron T. Wright |
Abstract |
The development of renewable biofuels is a global priority, but success will require novel technologies that greatly improve our understanding of microbial systems biology. An approach with great promise in enabling functional characterization of microbes is activity-based protein profiling (ABPP), which employs chemical probes to directly measure enzyme function in discrete enzyme classes in vivo and/or in vitro, thereby facilitating the rapid discovery of new biocatalysts and enabling much improved biofuel production platforms. We review general design strategies in ABPP, and highlight recent advances that are or could be pivotal to biofuels processes including applications of ABPP to cellulosic bioethanol, biodiesel, and phototrophic production of hydrocarbons. We also examine the key challenges and opportunities of ABPP in renewable biofuels research. The integration of ABPP with molecular and systems biology approaches will shed new insight on the catalytic and regulatory mechanisms of functional enzymes and their synergistic effects in the field of biofuels production. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Germany | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Bangladesh | 1 | 2% |
Thailand | 1 | 2% |
Unknown | 59 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 14 | 23% |
Researcher | 10 | 16% |
Student > Ph. D. Student | 10 | 16% |
Student > Master | 8 | 13% |
Other | 4 | 7% |
Other | 8 | 13% |
Unknown | 7 | 11% |
Readers by discipline | Count | As % |
---|---|---|
Chemistry | 12 | 20% |
Biochemistry, Genetics and Molecular Biology | 12 | 20% |
Agricultural and Biological Sciences | 11 | 18% |
Environmental Science | 9 | 15% |
Pharmacology, Toxicology and Pharmaceutical Science | 3 | 5% |
Other | 7 | 11% |
Unknown | 7 | 11% |