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Determining exon connectivity in complex mRNAs by nanopore sequencing

Overview of attention for article published in Genome Biology, September 2015
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About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (98th percentile)
  • High Attention Score compared to outputs of the same age and source (92nd percentile)

Mentioned by

news
9 news outlets
blogs
3 blogs
twitter
76 X users
googleplus
1 Google+ user
q&a
1 Q&A thread

Citations

dimensions_citation
136 Dimensions

Readers on

mendeley
283 Mendeley
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Title
Determining exon connectivity in complex mRNAs by nanopore sequencing
Published in
Genome Biology, September 2015
DOI 10.1186/s13059-015-0777-z
Pubmed ID
Authors

Mohan T. Bolisetty, Gopinath Rajadinakaran, Brenton R. Graveley

Abstract

Short-read high-throughput RNA sequencing, though powerful, is limited in its ability to directly measure exon connectivity in mRNAs that contain multiple alternative exons located farther apart than the maximum read length. Here, we use the Oxford Nanopore MinION sequencer to identify 7,899 'full-length' isoforms expressed from four Drosophila genes, Dscam1, MRP, Mhc, and Rdl. These results demonstrate that nanopore sequencing can be used to deconvolute individual isoforms and that it has the potential to be a powerful method for comprehensive transcriptome characterization.

X Demographics

X Demographics

The data shown below were collected from the profiles of 76 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 283 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 3 1%
Netherlands 2 <1%
Canada 2 <1%
Chile 1 <1%
Italy 1 <1%
Sweden 1 <1%
Czechia 1 <1%
Germany 1 <1%
France 1 <1%
Other 3 1%
Unknown 267 94%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 70 25%
Researcher 69 24%
Student > Master 34 12%
Student > Bachelor 28 10%
Professor > Associate Professor 14 5%
Other 39 14%
Unknown 29 10%
Readers by discipline Count As %
Agricultural and Biological Sciences 96 34%
Biochemistry, Genetics and Molecular Biology 88 31%
Computer Science 19 7%
Medicine and Dentistry 14 5%
Engineering 10 4%
Other 19 7%
Unknown 37 13%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 124. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 August 2017.
All research outputs
#339,894
of 25,658,139 outputs
Outputs from Genome Biology
#149
of 4,498 outputs
Outputs of similar age
#4,535
of 286,948 outputs
Outputs of similar age from Genome Biology
#6
of 84 outputs
Altmetric has tracked 25,658,139 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 98th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 4,498 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 27.6. This one has done particularly well, scoring higher than 96% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 286,948 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 98% of its contemporaries.
We're also able to compare this research output to 84 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 92% of its contemporaries.