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X Demographics
Mendeley readers
Attention Score in Context
| Title |
A Chlamydia trachomatis strain with a chemically generated amino acid substitution (P370L) in the cthtrA gene shows reduced elementary body production
|
|---|---|
| Published in |
BMC Microbiology, September 2015
|
| DOI | 10.1186/s12866-015-0533-2 |
| Pubmed ID | |
| Authors |
James W. Marsh, Bryan A. Wee, Joel D.A. Tyndall, William B. Lott, Robert J. Bastidas, Harlan D. Caldwell, Raphael H. Valdivia, L. Kari, Wilhelmina M. Huston |
| Abstract |
Chlamydia (C.) trachomatis is the most prevalent bacterial sexually transmitted infection worldwide and the leading cause of preventable blindness. Genetic approaches to investigate C. trachomatis have been only recently developed due to the organism's intracellular developmental cycle. HtrA is a critical stress response serine protease and chaperone for many bacteria and in C. trachomatis has been previously shown to be important for heat stress and the replicative phase of development using a chemical inhibitor of the CtHtrA activity. In this study, chemically-induced SNVs in the cthtrA gene that resulted in amino acid substitutions (A240V, G475E, and P370L) were identified and characterized. SNVs were initially biochemically characterized in vitro using recombinant protein techniques to confirm a functional impact on proteolysis. The C. trachomatis strains containing the SNVs with marked reductions in proteolysis were investigated in cell culture to identify phenotypes that could be linked to CtHtrA function. The strain harboring the SNV with the most marked impact on proteolysis (cthtrA P370L) was detected to have a significant reduction in the production of infectious elementary bodies. This provides genetic evidence that CtHtrA is critical for the C. trachomatis developmental cycle. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Australia | 1 | 33% |
| Unknown | 2 | 67% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 2 | 67% |
| Members of the public | 1 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 21 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 6 | 29% |
| Student > Ph. D. Student | 4 | 19% |
| Student > Doctoral Student | 3 | 14% |
| Student > Master | 3 | 14% |
| Other | 1 | 5% |
| Other | 3 | 14% |
| Unknown | 1 | 5% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 5 | 24% |
| Biochemistry, Genetics and Molecular Biology | 3 | 14% |
| Immunology and Microbiology | 3 | 14% |
| Nursing and Health Professions | 1 | 5% |
| Mathematics | 1 | 5% |
| Other | 4 | 19% |
| Unknown | 4 | 19% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 January 2016.
All research outputs
#16,104,877
of 24,503,376 outputs
Outputs from BMC Microbiology
#1,702
of 3,374 outputs
Outputs of similar age
#156,665
of 279,383 outputs
Outputs of similar age from BMC Microbiology
#35
of 79 outputs
Altmetric has tracked 24,503,376 research outputs across all sources so far. This one is in the 32nd percentile – i.e., 32% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,374 research outputs from this source. They receive a mean Attention Score of 4.2. This one is in the 44th percentile – i.e., 44% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 279,383 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 40th percentile – i.e., 40% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 79 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 50% of its contemporaries.