Title |
Determination of the infectious titer and virulence of an original US porcine epidemic diarrhea virus PC22A strain
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Published in |
Veterinary Research, September 2015
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DOI | 10.1186/s13567-015-0249-1 |
Pubmed ID | |
Authors |
Xinsheng Liu, Chun-Ming Lin, Thavamathi Annamalai, Xiang Gao, Zhongyan Lu, Malak A Esseili, Kwonil Jung, Mohamed El-Tholoth, Linda J Saif, Qiuhong Wang |
Abstract |
The infectious dose of a virus pool of original US PEDV strain PC22A was determined in 4-day-old, cesarean-derived, colostrum-deprived (CDCD) piglets. The median pig diarrhea dose (PDD50) of the virus pool was determined as 7.35 log10 PDD50/mL, similar to the cell culture infectious titer, 7.75 log10 plaque-forming units (PFU)/mL. 100 PDD50 caused watery diarrhea in all conventional suckling piglets (n = 12) derived from a PEDV-naive sow, whereas 1000 and 10 000 PDD50 did not cause diarrhea in piglets derived from two PEDV-field exposed-recovered sows. This information is important for future PEDV challenge studies and validation of PEDV vaccines. |
X Demographics
Geographical breakdown
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 52 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Master | 12 | 23% |
Researcher | 10 | 19% |
Other | 6 | 12% |
Student > Doctoral Student | 5 | 10% |
Student > Bachelor | 4 | 8% |
Other | 6 | 12% |
Unknown | 9 | 17% |
Readers by discipline | Count | As % |
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Veterinary Science and Veterinary Medicine | 10 | 19% |
Medicine and Dentistry | 8 | 15% |
Agricultural and Biological Sciences | 8 | 15% |
Biochemistry, Genetics and Molecular Biology | 4 | 8% |
Nursing and Health Professions | 2 | 4% |
Other | 7 | 13% |
Unknown | 13 | 25% |