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Quantification and characterization of Salmonella spp. isolates in sewage sludge with potential usage in agriculture

Overview of attention for article published in BMC Microbiology, October 2014
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Title
Quantification and characterization of Salmonella spp. isolates in sewage sludge with potential usage in agriculture
Published in
BMC Microbiology, October 2014
DOI 10.1186/s12866-014-0263-x
Pubmed ID
Authors

Flávio Krzyzanowski, Lincohn Zappelini, Solange Martone-Rocha, Milena Dropa, Maria Helena Matté, Flávia Nacache, Maria Tereza Pepe Razzolini

Abstract

This study aims to scrutinize Salmonella spp. and its serotypes in sewage sludge samples from wastewater treatment plants, and assesses the presence of virulence genes and antibiotics resistant to the profile. Samples (n = 54) were collected and analyzed in accordance with the EPA Method 1682/2006. For positive serological reaction, 40 strains were selected for PCR analyses and detection of spvC, invA and sseL virulence genes, plasmid presence and resistance to antibiotics. Salmonella spp. was detected in 38.9% of the samples collected (<0.006473 to 12.19 MPN/gTS). The most prevalent serotype was Salmonella Infantis. All Salmonella spp. (n = 35) presented at least one of the three virulence genes mentioned above and 40% harboured plasmids. Salmonella Typhimurium strains were isolated harbouring at least one of the following virulence genes: spvC, invA or sseL. Four Salmonella spp. isolates were resistant to tetracycline; three were resistant to trimethoprim-sulfamethoxazole, and one isolate was resistant to ciprofloxacin. Two Salmonella spp. strains presented multi resistance to antimicrobial agents. The results obtained demonstrated that Salmonella spp. have been found in sewage sludge, thus it is essential to set measures to mitigate human health risks when it is intended to be applied on agricultural soils.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 97 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Brazil 3 3%
Austria 1 1%
Armenia 1 1%
Unknown 92 95%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 18 19%
Student > Master 16 16%
Student > Doctoral Student 12 12%
Researcher 11 11%
Student > Bachelor 10 10%
Other 9 9%
Unknown 21 22%
Readers by discipline Count As %
Agricultural and Biological Sciences 27 28%
Environmental Science 15 15%
Immunology and Microbiology 7 7%
Engineering 5 5%
Veterinary Science and Veterinary Medicine 4 4%
Other 11 11%
Unknown 28 29%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 October 2015.
All research outputs
#20,294,248
of 22,830,751 outputs
Outputs from BMC Microbiology
#2,689
of 3,191 outputs
Outputs of similar age
#217,222
of 260,466 outputs
Outputs of similar age from BMC Microbiology
#48
of 57 outputs
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We're also able to compare this research output to 57 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.