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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Changes in DNA methylation are associated with the development of drug resistance in cervical cancer cells
|
|---|---|
| Published in |
Cancer Cell International, October 2015
|
| DOI | 10.1186/s12935-015-0248-3 |
| Pubmed ID | |
| Authors |
Chih-Cheng Chen, Kuan-Der Lee, Mei-Yu Pai, Pei-Yi Chu, Chia-Chen Hsu, Chia-Chen Chiu, Li-Tzong Chen, Jang-Yang Chang, Shu-Huei Hsiao, Yu-Wei Leu |
| Abstract |
Changes in DNA methylation are associated with changes in somatic cell fate without the alteration of coding sequences. In addition to its use as a traceable biomarker, reversible DNA methylation could also serve as a therapeutic target. In particular, if the development of drug resistance is associated with changes in DNA methylation, then demethylation might reverse the resistance phenotype. The reversion of the drug-resistance might then be feasible if the association between abnormal DNA methylation and the development of drug-resistance could be identified. Methylation differences between the drug-resistance cervical cancer cell, SiHa, and its derived oxaliplatin-resistant S3 cells were detected by methylation specific microarray. The drug-resistance cells were treated with demethylation agent to see if the resistance phenotype were reversed. Targeted methylation of one of the identified locus in normal cell is expected to recapitulate the development of resistance and a two-component reporter system is adopted to monitor the increase of DNA methylation in live cells. In this report, we identified methylation changes, both genome-wide and within individual loci, in the oxaliplatin-resistant cervical cancer cell S3 compared with its parental cell line SiHa. Treatment of S3 with a demethylation agent reversed increases in methylation and allowed the expression of methylation-silenced genes. Treatment with the demethylation agent also restored the sensitivity of S3 to cisplatin, taxol, and oxaliplatin to the same level as that of SiHa. Finally, we found that methylation of the target gene Casp8AP2 is sufficient to increase drug resistance in different cells. These results suggest that global methylation is associated with the development of drug resistance and could serve as a biomarker and therapeutic target for drug resistance in cervical cancer. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 50% |
| Practitioners (doctors, other healthcare professionals) | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 53 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Spain | 1 | 2% |
| Poland | 1 | 2% |
| Unknown | 51 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 11 | 21% |
| Student > Ph. D. Student | 8 | 15% |
| Student > Bachelor | 7 | 13% |
| Researcher | 7 | 13% |
| Student > Doctoral Student | 4 | 8% |
| Other | 7 | 13% |
| Unknown | 9 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 18 | 34% |
| Agricultural and Biological Sciences | 9 | 17% |
| Medicine and Dentistry | 7 | 13% |
| Immunology and Microbiology | 2 | 4% |
| Computer Science | 2 | 4% |
| Other | 4 | 8% |
| Unknown | 11 | 21% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 October 2015.
All research outputs
#17,775,656
of 22,830,751 outputs
Outputs from Cancer Cell International
#984
of 1,801 outputs
Outputs of similar age
#188,078
of 279,229 outputs
Outputs of similar age from Cancer Cell International
#13
of 24 outputs
Altmetric has tracked 22,830,751 research outputs across all sources so far. This one is in the 19th percentile – i.e., 19% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,801 research outputs from this source. They receive a mean Attention Score of 3.8. This one is in the 39th percentile – i.e., 39% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 279,229 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 28th percentile – i.e., 28% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 24 others from the same source and published within six weeks on either side of this one. This one is in the 33rd percentile – i.e., 33% of its contemporaries scored the same or lower than it.