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A genome-wide survey of mutations in the Jurkat cell line

Overview of attention for article published in BMC Genomics, May 2018
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About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (64th percentile)
  • Good Attention Score compared to outputs of the same age and source (78th percentile)

Mentioned by

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6 tweeters
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1 Facebook page
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1 research highlight platform

Citations

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25 Dimensions

Readers on

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114 Mendeley
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1 CiteULike
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Title
A genome-wide survey of mutations in the Jurkat cell line
Published in
BMC Genomics, May 2018
DOI 10.1186/s12864-018-4718-6
Pubmed ID
Authors

Louis Gioia, Azeem Siddique, Steven R. Head, Daniel R. Salomon, Andrew I. Su

Abstract

The Jurkat cell line has an extensive history as a model of T cell signaling. But at the turn of the 21st century, some expression irregularities were observed, raising doubts about how closely the cell line paralleled normal human T cells. While numerous expression deficiencies have been described in Jurkat, genetic explanations have only been provided for a handful of defects. Here, we report a comprehensive catolog of genomic variation in the Jurkat cell line based on whole-genome sequencing. With this list of all detectable, non-reference sequences, we prioritize potentially damaging mutations by mining public databases for functional effects. We confirm documented mutations in Jurkat and propose links from detrimental gene variants to observed expression abnormalities in the cell line. The Jurkat cell line harbors many mutations that are associated with cancer and contribute to Jurkat's unique characteristics. Genes with damaging mutations in the Jurkat cell line are involved in T-cell receptor signaling (PTEN, INPP5D, CTLA4, and SYK), maintenance of genome stability (TP53, BAX, and MSH2), and O-linked glycosylation (C1GALT1C1). This work ties together decades of molecular experiments and serves as a resource that will streamline both the interpretation of past research and the design of future Jurkat studies.

Twitter Demographics

The data shown below were collected from the profiles of 6 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 114 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 114 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 30 26%
Student > Bachelor 22 19%
Researcher 20 18%
Student > Master 16 14%
Professor > Associate Professor 6 5%
Other 9 8%
Unknown 11 10%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 49 43%
Agricultural and Biological Sciences 19 17%
Immunology and Microbiology 16 14%
Medicine and Dentistry 5 4%
Pharmacology, Toxicology and Pharmaceutical Science 4 4%
Other 9 8%
Unknown 12 11%

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 August 2019.
All research outputs
#4,845,662
of 16,649,395 outputs
Outputs from BMC Genomics
#2,489
of 9,094 outputs
Outputs of similar age
#99,486
of 280,829 outputs
Outputs of similar age from BMC Genomics
#3
of 14 outputs
Altmetric has tracked 16,649,395 research outputs across all sources so far. This one has received more attention than most of these and is in the 70th percentile.
So far Altmetric has tracked 9,094 research outputs from this source. They receive a mean Attention Score of 4.3. This one has gotten more attention than average, scoring higher than 72% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 280,829 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 64% of its contemporaries.
We're also able to compare this research output to 14 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 78% of its contemporaries.