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RIP3 dependent NLRP3 inflammasome activation is implicated in acute lung injury in mice

Overview of attention for article published in Journal of Translational Medicine, August 2018
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Title
RIP3 dependent NLRP3 inflammasome activation is implicated in acute lung injury in mice
Published in
Journal of Translational Medicine, August 2018
DOI 10.1186/s12967-018-1606-4
Pubmed ID
Authors

Jingxian Chen, Shuang Wang, Rong Fu, Mianjing Zhou, Tengyue Zhang, Wenxu Pan, Niansheng Yang, Yuefang Huang

Abstract

NLRP3 inflammasome is involved in the inflammatory responses during acute lung injury (ALI). RIP3 triggered NLRP3 inflammasome activation independent of necroptosis induction has recently been documented. In this study, the role of RIP3 in the activation of NLRP3 inflammasome in the development of ALI was investigated. A selective RIP3 inhibitor GSK872 was used to investigate the roles of RIP3 in NLRP3 inflammasome activation in the lipopolysaccharide (LPS) induced ALI mouse model. The mechanism of NLRP3 inflammasome activation was investigated in the human monocytic cell line THP-1. NLRP3 inflammasome and necroptosis were measured by flow cytometry or western blot. RIP3-NLRP3 interaction was interrogated using immunoprecipitation and the Duolink® In situ detection. Significant upregulation of both necroptosis and NLRP3 inflammasome pathways were observed in the lungs of mice with LPS induced ALI. GSK872 significantly suppressed the activation of necroptosis and NLRP3 activation with reduction of IL-1β and IL-18 production and inflammatory cells infiltration, resulting in a significant amelioration of lung injury. These two processes were shown to be active in interstitial macrophages and CD11b+ monocyte-macrophages/dendritic cells. In THP-1 cells, RIP3 and NLRP3 interaction was enhanced by LPS/ATP stimulation resulting in IL-1β and IL-18 production. This RIP3-NLRP3 interaction was significantly inhibited by GSK872. Taking together, these results show that RIP3 participates in the NLRP3 inflammasome activation in infiltrating macrophages in ALI induced by LPS. This process plays a significant pathogenic role in LPS-induced lung injury.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 28 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 28 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 5 18%
Student > Ph. D. Student 5 18%
Student > Bachelor 4 14%
Professor > Associate Professor 2 7%
Student > Master 2 7%
Other 2 7%
Unknown 8 29%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 6 21%
Medicine and Dentistry 6 21%
Neuroscience 2 7%
Agricultural and Biological Sciences 2 7%
Arts and Humanities 1 4%
Other 2 7%
Unknown 9 32%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 August 2018.
All research outputs
#10,664,944
of 13,406,897 outputs
Outputs from Journal of Translational Medicine
#2,190
of 2,644 outputs
Outputs of similar age
#200,330
of 268,023 outputs
Outputs of similar age from Journal of Translational Medicine
#1
of 1 outputs
Altmetric has tracked 13,406,897 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,644 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 7.1. This one is in the 9th percentile – i.e., 9% of its peers scored the same or lower than it.
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