Characterization of TaDREB1 in wheat genotypes with different seed germination under osmotic stress

Meng Liu, Zeng Wang, Hong-mei Xiao, Yan Yang

The cis-acting element DRE/CRT plays an important role in activating gene expression responsive to osmotic stress, low temperature and high-salinity. DREB1/CBF genes encode DRE-binding proteins with the function as transcript activators. TaDREB1 was also found to be induced by osmotic stress. The dates of osmotic stress was assessed by seed germination drought resistance index; the full-length cDNA sequences of TaDREB1 gene were downloaded from NCBI datebase; identification of allelic variation and transcript expression were assessed by PCR and semi-quantitive RT-PCR analysis, respectively. Total 13 new allele variations of TaDREB1 were identified in the germplasms tested in the paper, including 5 TaDREB1 -A on chromosome 3AL, 4 TaDREB1 -B on chromosome 3BL and 4 TaDREB1 -D on chromosome 3DL. In each variety, there existed two loci of TaDREB1-D genes, named TaDREB1-D1 and TaDREB1-D2, both of which had the similar nucleotide sequence except an 11 bp insertion in the former. In wheat seeds under osmotic stress, we did not detect the transcript expression level of TaDREB1-A and TaDREB1-B, but that of TaDREB1-D. The capacity of osmotic stress tolerance was closely correlated with the expression level and tendency of TaDREB1-D.