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Purification, characterization and immunogenicity assessment of glutaminase free L-asparaginase from Streptomyces brollosae NEAE-115

Overview of attention for article published in BMC Pharmacology and Toxicology, August 2018
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Title
Purification, characterization and immunogenicity assessment of glutaminase free L-asparaginase from Streptomyces brollosae NEAE-115
Published in
BMC Pharmacology and Toxicology, August 2018
DOI 10.1186/s40360-018-0242-1
Pubmed ID
Authors

Noura El-Ahmady El-Naggar, Sahar F. Deraz, Sara M. El-Ewasy, Ghada M. Suddek

Abstract

L-asparaginase is a potential therapeutic enzyme widely used in the chemotherapy protocols of pediatric and adult patients with acute lymphoblastic leukemia. However, its use has been limited by a high rate of hypersensitivity in the long-term used. Hence, there is a continuing need to search for other L-asparaginase sources capable of producing an enzyme with less adverse effects. Production of extracellular L-asparaginase by Streptomyces brollosae NEAE-115 was carried out using submerged fermentation. L-asparaginase was purified by ammonium sulphate precipitation and pure enzyme was reached using ion-exchange chromatography, followed by enzyme characterization. Anticancer activity towards Ehrlich Ascites Carcinoma (EAC) cells was investigated in female Swiss albino mice by determination of tumor size and the degree of tumor growth inhibition. The levels of anti-L-asparaginase IgG antibodies in mice sera were measured using ELISA method. The purified L-asparaginase showed a total activity of 795.152 with specific activity of 76.671 U/mg protein and 7.835 - purification fold. The enzyme purity was confirmed by using SDS-PAGE separation which revealed only one distinctive band with a molecular weight of 67 KDa. The enzyme showed maximum activity at pH 8.5, optimum temperature of 37 °C, incubation time of 50 min and optimum substrate concentration of 7 mM. A Michaelis-Menten constant analysis showed a Km value of 2.139 × 10- 3 M with L-asparagine as substrate and Vmax of 152.6 UmL- 1 min- 1. The half-life time (T1/2) was 65.02 min at 50°С, while being 62.65 min at 60°С. Furthermore, mice treated with Streptomyces brollosae NEAE-115 L-asparaginase showed higher cytotoxic effect (79% tumor growth inhibition) when compared to commercial L-asparaginase group (67% tumor growth inhibition). The study reveals the excellent property of this enzyme which makes it highly valuable for development of chemotherapeutic drug.

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Mendeley readers

The data shown below were compiled from readership statistics for 67 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 67 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 14 21%
Student > Bachelor 11 16%
Student > Doctoral Student 5 7%
Student > Master 5 7%
Other 4 6%
Other 10 15%
Unknown 18 27%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 24 36%
Agricultural and Biological Sciences 8 12%
Pharmacology, Toxicology and Pharmaceutical Science 5 7%
Immunology and Microbiology 3 4%
Engineering 2 3%
Other 5 7%
Unknown 20 30%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 August 2018.
All research outputs
#9,878,555
of 16,094,474 outputs
Outputs from BMC Pharmacology and Toxicology
#174
of 330 outputs
Outputs of similar age
#156,997
of 279,168 outputs
Outputs of similar age from BMC Pharmacology and Toxicology
#1
of 1 outputs
Altmetric has tracked 16,094,474 research outputs across all sources so far. This one is in the 36th percentile – i.e., 36% of other outputs scored the same or lower than it.
So far Altmetric has tracked 330 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.3. This one is in the 42nd percentile – i.e., 42% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 279,168 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 40th percentile – i.e., 40% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them